Literature summary for 1.1.1.44 extracted from

Cameron, S.; Martini, V.P.; Iulek, J.; Hunter, W.N.
Geobacillus stearothermophilus 6-phosphogluconate dehydrogenase complexed with 6-phosphogluconate (2009), Acta Crystallogr. Sect. F, 65, 450-454.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells	Geobacillus stearothermophilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
6-phosphogluconate dehydrogenase complexed with 6-phosphogluconate, hanging drop vapour diffusion method, using 0.2 M lithium sulfate, 2.2 M ammonium sulfate or 0.2 M ammonium acetate, 2.2 M ammonium sulfate	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography and Superdex 200 gel filtration	Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6-phospho-D-gluconate + NADP+	-	Geobacillus stearothermophilus	D-ribulose 5-phosphate + CO2 + NADPH + H+	-	?

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Geobacillus stearothermophilus

Synonyms

Synonyms	Comment	Organism
6-phosphogluconate dehydrogenase	-	Geobacillus stearothermophilus
6PDH	-	Geobacillus stearothermophilus

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Geobacillus stearothermophilus

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Release 2023.1 (January 2023)