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Literature summary for 1.1.1.44 extracted from

  • Sokolov, A.P.; Trotsenko, Y.A.
    Glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase from Arthrobacter globiformis (1990), Methods Enzymol., 188, 339-345.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
Arthrobacter globiformis
Co2+
-
Arthrobacter globiformis
Fe2+
-
Arthrobacter globiformis
NADH
-
Arthrobacter globiformis
NADPH
-
Arthrobacter globiformis
Ni2+
-
Arthrobacter globiformis
Zn2+
-
Arthrobacter globiformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
NADP+
-
Arthrobacter globiformis
0.11
-
6-phosphogluconate
-
Arthrobacter globiformis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Arthrobacter globiformis
Mg2+
-
Arthrobacter globiformis
Mn2+
-
Arthrobacter globiformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
4 * 57000, SDS-PAGE Arthrobacter globiformis
229000
-
gel filtration Arthrobacter globiformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-phospho-D-gluconate + NADP+ Arthrobacter globiformis
-
D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+ Arthrobacter globiformis VKM B-175
-
D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis
-
VKM B-175
-
Arthrobacter globiformis VKM B-175
-
VKM B-175
-

Purification (Commentary)

Purification (Comment) Organism
-
Arthrobacter globiformis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25.7
-
-
Arthrobacter globiformis

Storage Stability

Storage Stability Organism
4°C, purified enzyme in 50% glycerol buffer remains active for at least 3 months Arthrobacter globiformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NADP+
-
Arthrobacter globiformis D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+
-
Arthrobacter globiformis VKM B-175 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 57000, SDS-PAGE Arthrobacter globiformis

Cofactor

Cofactor Comment Organism Structure
NADP+ absolutely specific with respect to NADP+, no reaction can be detected in the presence of NAD+ Arthrobacter globiformis