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Literature summary for 1.1.1.44 extracted from
- 6-Phosphogluconate dehydrogenase from Neurospora crassa (1975), Methods Enzymol., 41, 227-231.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | - |
Neurospora crassa |  |
| NADPH | - |
Neurospora crassa | |
| phosphate | - |
Neurospora crassa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
6-phosphogluconate | - |
Neurospora crassa | |
| 0.03 | - |
NADP+ | - |
Neurospora crassa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
2 * 57000, SDS-PAGE | Neurospora crassa |
| 110000 | 120000 | gel filtration | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-phospho-D-gluconate + NADP+ | Saccharomyces cerevisiae | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 6-phospho-D-gluconate + NADP+ | Neurospora crassa | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Neurospora crassa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Neurospora crassa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30.8 | - |
- |
Neurospora crassa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, purified enzyme is stable up to 3 months if care is taken to exclude molecular oxygen from the enzyme solution | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-phospho-D-gluconate + NADP+ | - |
Saccharomyces cerevisiae | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 6-phospho-D-gluconate + NADP+ | - |
Neurospora crassa | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| additional information | neither gluconic acid nor glucose-6-phosphate can replace 6-phosphogluconate as substrate | Neurospora crassa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 57000, SDS-PAGE | Neurospora crassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 9 | - |
Neurospora crassa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | NAD+ is completely ineffective as hydrogen acceptor | Neurospora crassa | |
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Release 2023.1 (January 2023)
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