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Literature summary for 1.1.1.44 extracted from

  • Scott, W.A.; Abramsky, T.
    Neurospora 6-phosphogluconate dehydrogenase. II. Properties and two purified mutant enzymes (1973), J. Biol. Chem., 248, 3542-3545.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
6-phosphogluconate
-
Neurospora crassa
0.01
-
NADP+ wild-type, mutant col 10, heterocaryon col-10-wild-type Neurospora crassa
0.015
-
NADP+ heterocaryon col 3-wild-type Neurospora crassa
0.018
-
NADP+ heterocaryon col-3-col-10 Neurospora crassa
0.03
-
NADP+ mutant col-3 Neurospora crassa
0.04 0.05 6-phosphogluconate heterocaryon col-3-wild-type Neurospora crassa
0.05
-
6-phosphogluconate heterocaryon col-10-wild-type Neurospora crassa
0.06
-
6-phosphogluconate col-3-col-10 Neurospora crassa
0.09
-
6-phosphogluconate mutant col 3 Neurospora crassa
0.11
-
6-phosphogluconate mutant col 10 Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-phospho-D-gluconate + NADP+ Neurospora crassa enzyme specific for the oxidative pentose phosphate pathway D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type, mutant col-3, mutant col-10 Neurospora crassa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30
-
-
Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NADP+
-
Neurospora crassa D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+ enzyme specific for the oxidative pentose phosphate pathway Neurospora crassa D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r

Subunits

Subunits Comment Organism
dimer heteromultimeric molecule composed of 2 nonidentical subunits Neurospora crassa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
6-phosphogluconate increases heat stability Neurospora crassa