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Literature summary for 1.1.1.42 extracted from

  • Murugan, A.K.; Bojdani, E.; Xing, M.
    Identification and functional characterization of isocitrate dehydrogenase 1 (IDH1) mutations in thyroid cancer (2010), Biochem. Biophys. Res. Commun., 393, 555-559.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene idh1, expression of Myc-tagged wild-type and Myc-tagged mutant enzymes in HEK-293T cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
G123R site-directed mutagenesis, mutation is located in the catalytic domain, the mutant shows reduced activity compared to the wild-type enzyme Homo sapiens
R132H site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Homo sapiens
-
2-oxoglutarate + NADPH + H+ + CO2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens O75874 IDH1
-
Homo sapiens P48735 IDH2
-

Source Tissue

Source Tissue Comment Organism Textmining
B-CPAP cell
-
Homo sapiens
-
C-643 cell
-
Homo sapiens
-
FB-1 cell
-
Homo sapiens
-
FTC-133 cell
-
Homo sapiens
-
HTh-7 cell
-
Homo sapiens
-
HTh-74 cell
-
Homo sapiens
-
KAT-18 cell
-
Homo sapiens
-
OCUT-1 cell
-
Homo sapiens
-
OCUT-2 cell
-
Homo sapiens
-
SW-1736 cell
-
Homo sapiens
-
thyroid cancer cell
-
Homo sapiens
-
thyroid cancer cell line K1 cell Homo sapiens
-
thyroid cancer cell line K5 cell Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NADP+
-
Homo sapiens 2-oxoglutarate + NADPH + H+ + CO2
-
?

Synonyms

Synonyms Comment Organism
IDH1
-
Homo sapiens
IDH2
-
Homo sapiens
isocitrate dehydrogenase 1
-
Homo sapiens
isocitrate dehydrogenase 2
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Homo sapiens