Crystallization (Comment) | Organism |
---|---|
crystals of native DpIDH are obtained by the sitting-drop, vapour-diffusion method. The crystals are grown at 8°C. Crystals of DpIDH in complex with isocitrate (DpIDH-iso) are obtained by the hanging drop method at 8 °C | Desulfotalea psychrophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfotalea psychrophila | Q6AQ66 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Desulfotalea psychrophila | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DpIDH | - |
Desulfotalea psychrophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
melting temperature is 66.9°C | Desulfotalea psychrophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
NADP+ | 5°C | Desulfotalea psychrophila | |
4.5 | - |
isocitrate | 5°C | Desulfotalea psychrophila | |
31.4 | - |
NADP+ | 25°C | Desulfotalea psychrophila | |
31.4 | - |
isocitrate | 25°C | Desulfotalea psychrophila |