Protein Variants | Comment | Organism |
---|---|---|
D328K | the mutant shows dual coenzyme specificity | Pyrococcus furiosus |
D328K/I329Y | introduction of the double mutation shifts the cofactor preference from NAD+ to NADP+ | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.066 | - |
NAD+ | pH 8.0, 70°C, mutant enzyme D328K | Pyrococcus furiosus | |
0.068 | - |
NAD+ | pH 8.0, 70°C, wild-type enzyme | Pyrococcus furiosus | |
0.162 | - |
NADP+ | pH 8.0, 70°C, mutant enzyme D328K | Pyrococcus furiosus | |
0.211 | - |
NADP+ | pH 8.0, 70°C, mutant enzyme D328K/I329Y | Pyrococcus furiosus | |
0.336 | - |
NAD+ | pH 8.0, 70°C, mutant enzyme D328K/I329Y | Pyrococcus furiosus | |
4.44 | - |
NADP+ | pH 8.0, 70°C, wild-type enzyme | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U488 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | wild-type enzyme is specific for NAD+, low activity with NADP+ as cofactor, mutant D328K shows dual coenzyme specificity, cofactor preference of mutant D328K/I329Y is shifted from NAD+ to NADP+ | Pyrococcus furiosus | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NADP+ | wild-type enzyme is specific for NAD+, low activity with NADP+ as cofactor, mutant D328K shows dual coenzyme specificity, cofactor preference of mutant D328K/I329Y is shifted from NAD+ to NADP+ | Pyrococcus furiosus | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAD-IDH | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 95 | activity at 80°C is about 50% compared to the activity at 90°C | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
102 | - |
the apparent melting temperature is 102.3°C, mutant enzyme D328K | Pyrococcus furiosus |
103 | - |
the apparent melting temperature is 102.9°C, mutant enzyme D328K/I329Y | Pyrococcus furiosus |
104 | - |
the apparent melting temperature is 103.7°C, wild-type enzyme | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
60.4 | - |
NAD+ | pH 8.0, 70°C, wild-type enzyme | Pyrococcus furiosus | |
93 | - |
NADP+ | pH 8.0, 70°C, wild-type enzyme | Pyrococcus furiosus | |
103.4 | - |
NAD+ | pH 8.0, 70°C, mutant enzyme D328K | Pyrococcus furiosus | |
105.6 | - |
NAD+ | pH 8.0, 70°C, mutant enzyme D328K/I329Y | Pyrococcus furiosus | |
133 | - |
NADP+ | pH 8.0, 70°C, mutant enzyme D328K | Pyrococcus furiosus | |
168 | - |
NADP+ | pH 8.0, 70°C, mutant enzyme D328K/I329Y | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | wild-type enzyme is specific for NAD+, low activity with NADP+ as cofactor, mutant D328K shows dual coenzyme specificity, cofactor preference of mutant D328K/I329Y is shifted from NAD+ to NADP+ | Pyrococcus furiosus | |
NADP+ | wild-type enzyme is specific for NAD+, low activity with NADP+ as cofactor, mutant D328K shows dual coenzyme specificity, cofactor preference of mutant D328K/I329Y is shifted from NAD+ to NADP+ | Pyrococcus furiosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20.9 | - |
NADP+ | pH 8.0, 70°C, wild-type enzyme | Pyrococcus furiosus | |
314 | - |
NAD+ | pH 8.0, 70°C, mutant enzyme D328K/I329Y | Pyrococcus furiosus | |
796 | - |
NADP+ | pH 8.0, 70°C, mutant enzyme D328K/I329Y | Pyrococcus furiosus | |
821 | - |
NADP+ | pH 8.0, 70°C, mutant enzyme D328K | Pyrococcus furiosus | |
888 | - |
NAD+ | pH 8.0, 70°C, wild-type enzyme | Pyrococcus furiosus | |
1567 | - |
NAD+ | pH 8.0, 70°C, mutant enzyme D328K | Pyrococcus furiosus |