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Literature summary for 1.1.1.40 extracted from
- Plastidial NADP-malic enzymes from grasses: Unraveling the way to the C4 specific isoforms (2013), Plant Physiol. Biochem., 63, 39-48.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Zea mays |
| expressed in Escherichia coli BL21 cells | Sorghum bicolor |
| expressed in Escherichia coli BL21 cells | Oryza sativa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| malate | the enzyme is inhibited by high malate concentration at pH 7.0 | Oryza sativa |  |
| malate | the enzyme is inhibited by high malate concentration at pH 7.0 | Sorghum bicolor | |
| malate | the enzyme is inhibited by high malate concentration at pH 7.0 | Zea mays | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
NADP+ | at pH 8.0, temperature not specified in the publication | Zea mays | |
| 0.011 | - |
NADP+ | at pH 8.0, temperature not specified in the publication | Sorghum bicolor | |
| 0.035 | - |
NADP+ | at pH 8.0, temperature not specified in the publication | Oryza sativa | |
| 0.19 | - |
(S)-malate | at pH 8.0, temperature not specified in the publication | Zea mays | |
| 0.36 | - |
(S)-malate | at pH 8.0, temperature not specified in the publication | Sorghum bicolor | |
| 0.81 | - |
(S)-malate | at pH 8.0, temperature not specified in the publication | Oryza sativa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plastid | - |
Zea mays | 9536 | - |
| plastid | - |
Sorghum bicolor | 9536 | - |
| plastid | - |
Oryza sativa | 9536 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | Zea mays | - |
pyruvate + CO2 + NADPH | - |
? | |
| (S)-malate + NADP+ | Sorghum bicolor | - |
pyruvate + CO2 + NADPH | - |
? | |
| (S)-malate + NADP+ | Oryza sativa | - |
pyruvate + CO2 + NADPH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
- |
- |
| Sorghum bicolor | - |
- |
- |
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Zea mays |
| Ni-NTA column chromatography | Sorghum bicolor |
| Ni-NTA column chromatography | Oryza sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Zea mays | - |
| leaf | - |
Sorghum bicolor | - |
| leaf | - |
Oryza sativa | - |
| root | - |
Zea mays | - |
| root | - |
Sorghum bicolor | - |
| root | - |
Oryza sativa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | - |
Zea mays | pyruvate + CO2 + NADPH | - |
? | |
| (S)-malate + NADP+ | - |
Sorghum bicolor | pyruvate + CO2 + NADPH | - |
? | |
| (S)-malate + NADP+ | - |
Oryza sativa | pyruvate + CO2 + NADPH | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-malate:NADP oxidoreductase | - |
Zea mays |
| L-malate:NADP oxidoreductase | - |
Sorghum bicolor |
| L-malate:NADP oxidoreductase | - |
Oryza sativa |
| NADP-ME | - |
Zea mays |
| NADP-ME | - |
Sorghum bicolor |
| NADP-ME | - |
Oryza sativa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 181.6 | - |
(S)-malate | at pH 8.0, temperature not specified in the publication | Oryza sativa | |
| 198.9 | - |
(S)-malate | at pH 8.0, temperature not specified in the publication | Zea mays | |
| 275.7 | - |
(S)-malate | at pH 8.0, temperature not specified in the publication | Sorghum bicolor | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Zea mays | |
| NADP+ | - |
Sorghum bicolor | |
| NADP+ | - |
Oryza sativa | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 224.1 | - |
(S)-malate | at pH 8.0, temperature not specified in the publication | Oryza sativa | |
| 765.7 | - |
(S)-malate | at pH 8.0, temperature not specified in the publication | Sorghum bicolor | |
| 1047 | - |
(S)-malate | at pH 8.0, temperature not specified in the publication | Zea mays | |
| 5800 | - |
NADP+ | at pH 8.0, temperature not specified in the publication | Oryza sativa | |
| 27900 | - |
NADP+ | at pH 8.0, temperature not specified in the publication | Sorghum bicolor | |
| 61200 | - |
NADP+ | at pH 8.0, temperature not specified in the publication | Zea mays | |
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