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Literature summary for 1.1.1.40 extracted from

  • Xue, J.; Wang, L.; Zhang, L.; Balamurugan, S.; Li, D.; Zeng, H.; Yang, W.; Liu, J.; Li, H.
    The pivotal role of malic enzyme in enhancing oil accumulation in green microalga Chlorella pyrenoidosa (2016), Microb. Cell Fact., 15, 120.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
biotechnology malic enzyme is a pivotal regulator in lipid accumulation in green microalga Chlorella pyrenoidosa, and presents a breakthrough of generating ideal algal strains for algal nutrition and biofuels Auxenochlorella pyrenoidosa

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Chlorella pyrenoidosa Phaeodactylum tricornutum
sequence comparisons, recombinant enzyme overexpression in Chlorella pyrenoidosa Auxenochlorella pyrenoidosa

Protein Variants

Protein Variants Comment Organism
additional information recombinant enzyme overexpression in Chlorella pyrenoidosa for enhanced lipid production, method, overview Auxenochlorella pyrenoidosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60400
-
x * 60400, calculated from amino acid sequence Phaeodactylum tricornutum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NADP+ Auxenochlorella pyrenoidosa
-
pyruvate + CO2 + NADPH
-
r
(S)-malate + NADP+ Phaeodactylum tricornutum
-
pyruvate + CO2 + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Auxenochlorella pyrenoidosa
-
-
-
Phaeodactylum tricornutum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Auxenochlorella pyrenoidosa pyruvate + CO2 + NADPH
-
r
(S)-malate + NADP+
-
Phaeodactylum tricornutum pyruvate + CO2 + NADPH + H+
-
?

Subunits

Subunits Comment Organism
? x * 60400, calculated from amino acid sequence Phaeodactylum tricornutum

Synonyms

Synonyms Comment Organism
malic enzyme
-
Auxenochlorella pyrenoidosa
NADP-malic enzyme
-
Auxenochlorella pyrenoidosa
NADP-malic enzyme
-
Phaeodactylum tricornutum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Auxenochlorella pyrenoidosa
NADP+
-
Phaeodactylum tricornutum
NADPH
-
Auxenochlorella pyrenoidosa

General Information

General Information Comment Organism
additional information fatty acid composition analysis of wild-type and mutant overexpressing strains, overview Auxenochlorella pyrenoidosa
physiological function pivotal role of malic enzyme in enhancing oil accumulation in green microalga Chlorella pyrenoidosa. The enzyme promte fatty acid biosynthesis Auxenochlorella pyrenoidosa