Literature summary for 1.1.1.40 extracted from

Hao, G.; Chen, H.; Wang, L.; Gu, Z.; Song, Y.; Zhang, H.; Chen, W.; Chen, Y.Q.
Role of malic enzyme during fatty acid synthesis in the oleaginous fungus Mortierella alpina (2014), Appl. Environ. Microbiol., 80, 2672-2678.

Cloned(Commentary)

Cloned (Comment)	Organism
gene malE1, recombinant overexpression of the gene encoding ME isoform E from Mortierella alpina via Agrobacterium tumefaciens-mediated transformation in Mortierella alpina uracil auxotrophic strain increasing the fatty acid content by 30% compared to that for wild-type control, quantitative reverse transcription PCR enzyme expression analysis. The expression of the malE1 gene in three randomly selected malE1-overexpressing strains	Mortierella alpina

Cloned (Comment)

Organism

gene malE1, recombinant overexpression of the gene encoding ME isoform E from Mortierella alpina via Agrobacterium tumefaciens-mediated transformation in Mortierella alpina uracil auxotrophic strain increasing the fatty acid content by 30% compared to that for wild-type control, quantitative reverse transcription PCR enzyme expression analysis. The expression of the malE1 gene in three randomly selected malE1-overexpressing strains

Mortierella alpina

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Mortierella alpina	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	activates	Mortierella alpina

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
(S)-malate + NADP+	Mortierella alpina	-	pyruvate + CO2 + NADPH	-	?
(S)-malate + NADP+	Mortierella alpina	-	pyruvate + CO2 + NADPH	-	r
(S)-malate + NADP+	Mortierella alpina ATCC 32222	-	pyruvate + CO2 + NADPH	-	?
(S)-malate + NADP+	Mortierella alpina ATCC 32222	-	pyruvate + CO2 + NADPH	-	r

Organism

Organism	UniProt	Comment	Textmining
Mortierella alpina	-	-	-
Mortierella alpina	A6XP71	-	-
Mortierella alpina ATCC 32222	-	-	-
Mortierella alpina ATCC 32222	A6XP71	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
mycelium	-	Mortierella alpina	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
(S)-malate + NADP+	-	Mortierella alpina	pyruvate + CO2 + NADPH	-	?
(S)-malate + NADP+	-	Mortierella alpina	pyruvate + CO2 + NADPH	-	r
(S)-malate + NADP+	-	Mortierella alpina ATCC 32222	pyruvate + CO2 + NADPH	-	?
(S)-malate + NADP+	-	Mortierella alpina ATCC 32222	pyruvate + CO2 + NADPH	-	r

Synonyms

Synonyms	Comment	Organism
malE1	-	Mortierella alpina
malic enzyme	-	Mortierella alpina
ME1	-	Mortierella alpina

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Mortierella alpina

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Mortierella alpina

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Mortierella alpina
NADPH	-	Mortierella alpina

General Information

General Information	Comment	Organism
metabolism	the enzyme plays a role during fatty acid synthesis	Mortierella alpina
physiological function	NADP+-malic enzyme may not be the sole rate-limiting enzyme, but does play a role, during fatty acid synthesis in oleaginous fungi	Mortierella alpina