Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.40 extracted from

  • Yang, J.; Hu, X.; Zhang, H.; Chen, H.; Kargbo, M.R.; Zhao, J.; Song, Y.; Chen, Y.Q.; Zhang, H.; Chen, W.
    Expression, purification, and characterization of NADP+-dependent malic enzyme from the oleaginous fungus Mortierella alpina (2014), Appl. Biochem. Biotechnol., 173, 1849-1857.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mortierella alpina

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ complete inhibition at 5 mM Mortierella alpina
Co2+ about 90% activity at 0.5 mM Mortierella alpina
Cu2+ complete inhibition at 5 mM Mortierella alpina
glyoxylate competitive inhibition, about 60% activity at 2 mM Mortierella alpina
Mg2+ about 80% activity at 0.5 mM Mortierella alpina
additional information not inhibited by ATP, ADP, AMP, propionyl-COA, acetyl-CoA, CoA, succinate, L-glutamate, L-aspartate, isocitrate, citrate, pyruvate, D-fructose 6-phosphate, D-glucose 6-phosphate, and 2-oxoglutarate Mortierella alpina
Ni2+ about 50% activity at 0.5 mM Mortierella alpina
oxaloacetate competitive inhibition, about 25% activity at 2 mM Mortierella alpina
Zn2+ about 10% activity at 5 mM Mortierella alpina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
NADP+ at pH 7.5 and 33°C Mortierella alpina
2.19
-
(S)-malate at pH 7.5 and 33°C Mortierella alpina

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ about 115% activity at 5 mM Mortierella alpina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
x * 64000, SDS-PAGE Mortierella alpina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NADP+ Mortierella alpina
-
pyruvate + CO2 + NADPH
-
?
(S)-malate + NADP+ Mortierella alpina ATCC 32222
-
pyruvate + CO2 + NADPH
-
?

Organism

Organism UniProt Comment Textmining
Mortierella alpina
-
-
-
Mortierella alpina ATCC 32222
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Mortierella alpina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Mortierella alpina pyruvate + CO2 + NADPH
-
?
(S)-malate + NADP+
-
Mortierella alpina ATCC 32222 pyruvate + CO2 + NADPH
-
?

Subunits

Subunits Comment Organism
? x * 64000, SDS-PAGE Mortierella alpina

Synonyms

Synonyms Comment Organism
malE1
-
Mortierella alpina
NADP+-dependent malic enzyme
-
Mortierella alpina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
33
-
the enzyme is completely inactivated above 45°C Mortierella alpina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Mortierella alpina

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Mortierella alpina

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.08
-
oxaloacetate at pH 7.5 and 33°C Mortierella alpina
0.6
-
glyoxylate at pH 7.5 and 33°C Mortierella alpina