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Literature summary for 1.1.1.40 extracted from
- Loss of cytosolic NADP-malic enzyme 2 in Arabidopsis thaliana is associated with enhanced susceptibility to Colletotrichum higginsianum (2012), New Phytol., 195, 189-202.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genes NADP-ME1, NADP-ME2, and NADP-ME3, quantitative RT-PCR expression analysis | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of knock-out mutants nadp-me2.1 and -2.2 by Agrobacterium tumefaciens strain GV3101-mediated transformation using the vacuum infiltration method | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | NADP-malic enzyme 2 | Arabidopsis thaliana | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Arabidopsis thaliana |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | Arabidopsis thaliana | - |
pyruvate + CO2 + NADPH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
isozymes NADP-ME1, NADP-ME2, and NADP-ME3 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Arabidopsis thaliana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
relative NADP-malic enzyme activity in rosette leaves of Arabidopsis thaliana wild-type, loss-of-function (nadp-me2.1 and -2.2), complemented (nadp-me2.1 ? ME2-1 and -2) and overexpressing (ME2-1 and -2) lines, overview | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | - |
Arabidopsis thaliana | pyruvate + CO2 + NADPH | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADP-malic enzyme | - |
Arabidopsis thaliana |
| NADP-malic enzyme 2 | - |
Arabidopsis thaliana |
| NADP-ME | - |
Arabidopsis thaliana |
| NADP-ME1 | - |
Arabidopsis thaliana |
| NADP-ME2 | - |
Arabidopsis thaliana |
| NADP-ME3 | - |
Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Arabidopsis thaliana | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | loss of cytosolic NADP-malic enzyme 2 in Arabidopsis thaliana is associated with enhanced susceptibility to Colletotrichum higginsianum, transient apoplastic reactive oxygen species production after elicitation and callose papilla formation after infection are dampened in mutant nadp-me2 | Arabidopsis thaliana |
| additional information | analysis of transcriptional co-response patterns related NADP-ME2 to plant defence responses, overview | Arabidopsis thaliana |
| physiological function | ADP-ME2 is an important player in plant basal defence, where it is involved in the generation of reactive oxygen species, NADP-ME2 is dispensable for later defence responses, overview | Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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