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Literature summary for 1.1.1.40 extracted from

  • Heart, E.; Cline, G.W.; Collis, L.P.; Pongratz, R.L.; Gray, J.P.; Smith, P.J.
    Role for malic enzyme, pyruvate carboxylation, and mitochondrial malate import in glucose-stimulated insulin secretion (2009), Am. J. Physiol. Endocrinol. Metab., 296, E1354-E1362.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.40

Cloned(Commentary)

Cloned (Comment) Organism
functional expression of ME1, under the control of cytomegalovirus, which also directs the transcription of GFP from an internal ribosome entry site, in Rattus norvegicus INS-1 832/13 cells and in Mus musculus islets Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information ME1 overexpression results in an increased glucose-dependent rise in malate and citrate levels in INS-1 832/13 cells. Introduction of ME1 activity alters glucose-stimulated insulin secretion to a lesser degree in mouse islets than in INS-1 832/13 cells, overview. In contrast to rat, mouse beta-cells lack ME1 activity, which is suggested to explain their lack of methyl succinate-stimulated insulin secretion. Metabolic phenotypes of transfected cells, overview Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
 (S)-malate recombinant ME1, pH 7.4, temperature not specified in the publication Homo sapiens
5.8
-
 pyruvate recombinant ME1, pH 7.4, temperature not specified in the publication Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NADP+ Homo sapiens
-
 pyruvate + NADPH + H+ + CO2
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant ME1 2229fold from INS-1 832/13 cell cytosolic fractions by 2',5'-ADP affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
pancreatic beta cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.92
-
 recombinant enzyme in INS-1 832/13 cells, reverse reaction, pH 7.4, temperature not specified in the publication Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
 Homo sapiens pyruvate + NADPH + H+ + CO2
-
 r

Synonyms

Synonyms Comment Organism
cytosolic malic enzyme
-
 Homo sapiens
malic enzyme
-
 Homo sapiens
ME1
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Homo sapiens
NADPH
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function some pyruvate cycling pathways require malate export from mitochondria and NADP+-dependent decarboxylation of malate to pyruvate by cytosolic malic enzyme ME1. Role of ME1 in glucose-stimulated insulin secretion and in methyl succinate-stimulated insulin secretion occuring occur via succinate entry into the mitochondria in exchange for malate and subsequent malate conversion to pyruvate, overview Homo sapiens