Any feedback?
Literature summary for 1.1.1.40 extracted from
- Malic enzyme: the controlling activity for lipid production? Overexpression of malic enzyme in Mucor circinelloides leads to a 2.5-fold increase in lipid accumulation (2007), Microbiology, 153, 2013-2025.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene malE, DNA and amino acid sequence determination and analysis, genomic library construction, screening, and functional enzyme expression in Escherichia coli | Mucor circinelloides |
| gene malE, DNA and amino acid sequence determination and analysis, genomic library construction, screening, and functional enzyme expression in Escherichia coli | Mortierella alpina |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | Mucor circinelloides | the enzyme is rate-limiting step for fatty acid biosynthesis in oleaginous fungi in which the extent of lipid accumulation is below the maximum possible, overview | pyruvate + CO2 + NADPH | - |
r |  |
| (S)-malate + NADP+ | Mortierella alpina | the enzyme is rate-limiting step for fatty acid biosynthesis in oleaginous fungi in which the extent of lipid accumulation is below the maximum possible, overview | pyruvate + CO2 + NADPH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mortierella alpina | A6XP71 | a commercially useful oil-producing fungus, strain CBS 696.70, gene malE | - |
| Mucor circinelloides | A6XP72 | a commercially useful oil-producing fungus, strain CBS 108.16, gene malE | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | - |
Mucor circinelloides | pyruvate + CO2 + NADPH | - |
r | |
| (S)-malate + NADP+ | - |
Mortierella alpina | pyruvate + CO2 + NADPH | - |
r | |
| (S)-malate + NADP+ | the enzyme is rate-limiting step for fatty acid biosynthesis in oleaginous fungi in which the extent of lipid accumulation is below the maximum possible, overview | Mucor circinelloides | pyruvate + CO2 + NADPH | - |
r | |
| (S)-malate + NADP+ | the enzyme is rate-limiting step for fatty acid biosynthesis in oleaginous fungi in which the extent of lipid accumulation is below the maximum possible, overview | Mortierella alpina | pyruvate + CO2 + NADPH | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| malic enzyme | - |
Mucor circinelloides |
| malic enzyme | - |
Mortierella alpina |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Mucor circinelloides |
| 7.5 | - |
assay at | Mortierella alpina |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Mucor circinelloides | |
| NADP+ | - |
Mortierella alpina | |
| NADPH | - |
Mucor circinelloides | |
| NADPH | - |
Mortierella alpina | |
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Release 2023.1 (January 2023)
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