Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Columba sp. |
Crystallization (Comment) | Organism |
---|---|
10 mg/ml purified enzyme in quarternary complex with NADP+, Mn2+, and oxalate, closed enzyme form, hanging drop vapour diffusion method, 4°C, from 20 mM Tris, pH 7.4, 10 mM oxalate, 5 mM MnCl2, 0.23 mM NADP+, and 2 mM DTT, with a reservoir solution containing 100 mM sodium citrate, pH 5.5, 8% PEG 6000, and 1 M LiCl, 3-4 days, X-ray diffraction structure determination and analysis at 2.5 A resolution, atomic model | Columba sp. |
Protein Variants | Comment | Organism |
---|---|---|
K362A | site-directed mutagenesis, 70fold increased Km for NADP+ compared to the wild-type enzyme | Columba sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Columba sp. | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Columba sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Columba sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by pecipitation with streptomycin sulfate, DEAE ion exchange, affinity and gel filtration | Columba sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Columba sp. | pyruvate + CO2 + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | subunit structure and organisation, crystal structure | Columba sp. |
Synonyms | Comment | Organism |
---|---|---|
malic enzyme | - |
Columba sp. |
ME | - |
Columba sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Columba sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | dependent on, 1 cofactor molecule per subunit, binding mode, structure around the binding site at the 2'-phosphate group helps to define the cofactor specificity of the enzyme, Lys362 and Ser346 are involved, molecular mechanism | Columba sp. |