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Literature summary for 1.1.1.4 extracted from

  • Wang, Y.; Tao, F.; Xu, P.
    Glycerol dehydrogenase plays a dual role in glycerol metabolism and 2,3-butanediol formation in Klebsiella pneumoniae (2014), J. Biol. Chem., 289, 6080-6090.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.15
-
(3R,3S)-butane-2,3-diol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae
4.94
-
meso-butane-2,3-diol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae
12.06
-
(3R,3S)-butane-2,3-diol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
14.49
-
glycerol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae
37.39
-
meso-butane-2,3-diol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
93.62
-
glycerol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
119.3
-
(2R,3R)-butane-2,3-diol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae
236.4
-
(2R,3R)-butane-2,3-diol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, SDS-PAGE Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R,3R)-butane-2,3-diol + NAD+ Klebsiella pneumoniae
-
(3R)-acetoin + NADH + H+
-
?
(2R,3R)-butane-2,3-diol + NAD+ Klebsiella pneumoniae ATCC 25955
-
(3R)-acetoin + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae ATCC 25955
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Sephadex G-25 gel filtration Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3R)-butane-2,3-diol + NAD+
-
Klebsiella pneumoniae (3R)-acetoin + NADH + H+
-
?
(2R,3R)-butane-2,3-diol + NAD+
-
Klebsiella pneumoniae ATCC 25955 (3R)-acetoin + NADH + H+
-
?
(3R,3S)-butane-2,3-diol + NAD+
-
Klebsiella pneumoniae (3R,3S)-acetoin + NADH + H+
-
?
(3R,3S)-butane-2,3-diol + NAD+
-
Klebsiella pneumoniae ATCC 25955 (3R,3S)-acetoin + NADH + H+
-
?
glycerol + NAD+
-
Klebsiella pneumoniae ?
-
?
glycerol + NAD+
-
Klebsiella pneumoniae ATCC 25955 ?
-
?
meso-butane-2,3-diol + NAD+
-
Klebsiella pneumoniae (3S)-acetoin + NADH + H+
-
?
meso-butane-2,3-diol + NAD+
-
Klebsiella pneumoniae ATCC 25955 (3S)-acetoin + NADH + H+
-
?
additional information no activity with (2S,3S)-butane-2,3-diol or diacetyl Klebsiella pneumoniae ?
-
?
additional information no activity with (2S,3S)-butane-2,3-diol or diacetyl Klebsiella pneumoniae ATCC 25955 ?
-
?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Klebsiella pneumoniae

Synonyms

Synonyms Comment Organism
DHAD
-
Klebsiella pneumoniae
GldA
-
Klebsiella pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.68
-
(3R,3S)-butane-2,3-diol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
3.82
-
(2R,3R)-butane-2,3-diol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae
4.67
-
meso-butane-2,3-diol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
5.8
-
(2R,3R)-butane-2,3-diol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
10.38
-
(3R,3S)-butane-2,3-diol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae
10.51
-
glycerol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
15.33
-
meso-butane-2,3-diol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae
18.88
-
glycerol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Klebsiella pneumoniae

Expression

Organism Comment Expression
Klebsiella pneumoniae the enzyme gene dhaD is highly induced by glycerol up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.03
-
(2R,3R)-butane-2,3-diol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
0.03
-
(2R,3R)-butane-2,3-diol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae
0.11
-
glycerol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
0.13
-
meso-butane-2,3-diol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
0.31
-
(3R,3S)-butane-2,3-diol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
1.3
-
glycerol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae
2.51
-
(3R,3S)-butane-2,3-diol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae
3.11
-
meso-butane-2,3-diol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae