General Stability | Organism |
---|---|
EDTA has no effect enzyme thermal stability | [Clostridium] innocuum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-oxo bile acids | the specific enzyme activity is significantly reduced by growth medium supplementation with 3-oxo bile acids, with trisubstituted acids being more effective than disubstituted ones | [Clostridium] innocuum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetis | [Clostridium] innocuum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound | [Clostridium] innocuum | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
gel filtration | [Clostridium] innocuum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+ | [Clostridium] innocuum | - |
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+ | - |
? | |
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+ | [Clostridium] innocuum D1144/3 | - |
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+ | - |
? | |
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | [Clostridium] innocuum | - |
3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+ | - |
? | |
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | [Clostridium] innocuum D1144/3 | - |
3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Clostridium] innocuum | - |
- |
- |
[Clostridium] innocuum D1144/3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 6.3fold by anion exchange chromatography and gel filtration | [Clostridium] innocuum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the enzyme production is constitutive in the absence of added bile acids | [Clostridium] innocuum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.2 | - |
purified enzyme, pH 10.0-10.2, 37°C | [Clostridium] innocuum |
Storage Stability | Organism |
---|---|
4°C, crude enzyme extract, pH 8.5, in the presence of dithiothreitol, no loss of activity after 24 h | [Clostridium] innocuum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
[Clostridium] innocuum | 3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+ | sole pruduct | ? | |
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
[Clostridium] innocuum D1144/3 | 3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+ | sole pruduct | ? | |
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+ | - |
[Clostridium] innocuum | 3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+ | - |
? | |
3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+ | - |
[Clostridium] innocuum D1144/3 | 3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+ | - |
? | |
3,12-dioxo-5beta-cholan-24-oic acid + NADH + H+ | - |
[Clostridium] innocuum | 3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+ | - |
? | |
3,12-dioxo-5beta-cholan-24-oic acid + NADH + H+ | - |
[Clostridium] innocuum D1144/3 | 3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+ | - |
? | |
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
[Clostridium] innocuum | 3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+ | - |
? | |
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
[Clostridium] innocuum D1144/3 | 3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+ | - |
? | |
7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
[Clostridium] innocuum | 3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+ | - |
? | |
additional information | the enzyme reduces a 3-oxo group in unconjugated and conjugated bile acids. Lower Km values are demonstrated with disubstituted than with trisubstituted bile acids, oxo functions at C-7 and C-12 further reduce the Km value | [Clostridium] innocuum | ? | - |
? | |
additional information | the enzyme reduces a 3-oxo group in unconjugated and conjugated bile acids. Lower Km values are demonstrated with disubstituted than with trisubstituted bile acids, oxo functions at C-7 and C-12 further reduce the Km value | [Clostridium] innocuum D1144/3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 56000, SDS-PAGE | [Clostridium] innocuum |
Synonyms | Comment | Organism |
---|---|---|
3beta-HSD | - |
[Clostridium] innocuum |
NAD-dependent 3beta-hydroxysteroid dehydrogenase | - |
[Clostridium] innocuum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | [Clostridium] innocuum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
crude enzyme extract, pH 8.5, inactivation after 30 min | [Clostridium] innocuum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | 10.02 | - |
[Clostridium] innocuum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on | [Clostridium] innocuum |
Organism | Comment | Expression |
---|---|---|
[Clostridium] innocuum | effect of bile acids on the production of 3beta-HSDH activity in Clostridium innocuum strain D1144/3 | down |