Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.39 extracted from

  • Hasan, N.M.; Longacre, M.J.; Stoker, S.W.; Kendrick, M.A.; MacDonald, M.J.
    Mitochondrial malic enzyme 3 is important for insulin secretion in pancreatic beta-cells (2015), Mol. Endocrinol., 29, 396-410.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene ME1, quantitative RT-PCR expression analysis of isozymes Me1, Me2, and Me3 in wild-type strain and mutant Me1 strains Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information generation of Me1 single and double knockdown cell lines, thereby generating cell line 753(H)/Me1-753(P), overview. The level of Me1 mRNA in the double-knockdown cell line Me1-753(H)/Me1-753(P) is decreased to 2% as compared with 14% in the single knockdown Me1-753(H) cell line. The level of Me2mRNAis lowered by only 10% in the Me1-753(H) cell line and Me3 mRNA is lowered by only 27% in the Me1-753(H) cell line and lowered by only 35% in the Me1-753(H)/Me1-753(P) cell line. Resident siRNA might interfere with the expression of the siRNA expressed from the second vector. Knockdown of isozyme ME3, EC 1.1.1.40, but not ME1 or ME2 (both EC 1.1.1.39) alone or together, inhibits insulin release stimulated by glucose, pyruvate or 2-aminobicyclo [2,2,1]heptane-2-carboxylic acid-plus-glutamine. In the Me3 double-knockdown cells, the level of Me1 mRNA is not significantly decreased in the Me3-628(P)/Me2-725(H) and Me3-1672(P)/Me2-725(H) cell lines. However, a 32%, 49%, and 47% decrease in ME1 activity is observed in the cell lines Me3-628(P), Me3-628(P)/Me2-725(H), and Me3-628(P)/Me2-2124(H), respectively Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus norvegicus 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Rattus norvegicus
-
pyruvate + CO2 + NADH
-
r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
INS-1 832/13 cell
-
Rattus norvegicus
-
insulinoma cell
-
Rattus norvegicus
-
pancreas
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Rattus norvegicus pyruvate + CO2 + NADH
-
r

Synonyms

Synonyms Comment Organism
cytosolic malic enzyme
-
Rattus norvegicus
ME1
-
Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rattus norvegicus
NADH
-
Rattus norvegicus

General Information

General Information Comment Organism
malfunction knockdown of ME1 does not inhibit insulin release stimulated by glucose, pyruvate or 2-aminobicyclo [2,2,1]heptane-2-carboxylic acid-plus-glutamine Rattus norvegicus