Cloned (Comment) | Organism |
---|---|
gene ME1, quantitative RT-PCR expression analysis of isozymes Me1, Me2, and Me3 in wild-type strain and mutant Me1 strains | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of Me1 single and double knockdown cell lines, thereby generating cell line 753(H)/Me1-753(P), overview. The level of Me1 mRNA in the double-knockdown cell line Me1-753(H)/Me1-753(P) is decreased to 2% as compared with 14% in the single knockdown Me1-753(H) cell line. The level of Me2mRNAis lowered by only 10% in the Me1-753(H) cell line and Me3 mRNA is lowered by only 27% in the Me1-753(H) cell line and lowered by only 35% in the Me1-753(H)/Me1-753(P) cell line. Resident siRNA might interfere with the expression of the siRNA expressed from the second vector. Knockdown of isozyme ME3, EC 1.1.1.40, but not ME1 or ME2 (both EC 1.1.1.39) alone or together, inhibits insulin release stimulated by glucose, pyruvate or 2-aminobicyclo [2,2,1]heptane-2-carboxylic acid-plus-glutamine. In the Me3 double-knockdown cells, the level of Me1 mRNA is not significantly decreased in the Me3-628(P)/Me2-725(H) and Me3-1672(P)/Me2-725(H) cell lines. However, a 32%, 49%, and 47% decrease in ME1 activity is observed in the cell lines Me3-628(P), Me3-628(P)/Me2-725(H), and Me3-628(P)/Me2-2124(H), respectively | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Rattus norvegicus | - |
pyruvate + CO2 + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
INS-1 832/13 cell | - |
Rattus norvegicus | - |
insulinoma cell | - |
Rattus norvegicus | - |
pancreas | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Rattus norvegicus | pyruvate + CO2 + NADH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
cytosolic malic enzyme | - |
Rattus norvegicus |
ME1 | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rattus norvegicus | |
NADH | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
malfunction | knockdown of ME1 does not inhibit insulin release stimulated by glucose, pyruvate or 2-aminobicyclo [2,2,1]heptane-2-carboxylic acid-plus-glutamine | Rattus norvegicus |