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Literature summary for 1.1.1.39 extracted from

  • Rodriguez, E.; Navone, L.; Casati, P.; Gramajo, H.
    Impact of malic enzymes on antibiotic and triacylglycerol production in Streptomyces coelicolor (2012), Appl. Environ. Microbiol., 78, 4571-4579.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.39

Cloned(Commentary)

Cloned (Comment) Organism
gene Sco2951, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expressionin Escherichia coli strain BL21(DE3) Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3
-
 NAD+ pH 8.0, 30°C, recombinant enzyme Streptomyces coelicolor
8.1
-
 (S)-malate pH 8.0, 30°C, recombinant enzyme Streptomyces coelicolor

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Streptomyces coelicolor
-
 pyruvate + CO2 + NADH
-
 ?
(S)-malate + NAD+ Streptomyces coelicolor M145
-
 pyruvate + CO2 + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
 gene Sco2951
-
Streptomyces coelicolor M145
-
 gene Sco2951
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
 Streptomyces coelicolor pyruvate + CO2 + NADH
-
 ?
(S)-malate + NAD+
-
 Streptomyces coelicolor M145 pyruvate + CO2 + NADH
-
 ?

Synonyms

Synonyms Comment Organism
malic enzyme-NAD
-
 Streptomyces coelicolor
ME-NAD
-
 Streptomyces coelicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Streptomyces coelicolor

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on Streptomyces coelicolor

General Information

General Information Comment Organism
malfunction the single Sco2951 and the double Sco2951 Sco5261 mutants, deficient in ME-NAD and ME-NADP, EC 1.1.1.40, activity, display a strong reduction in the production of the polyketide antibiotic actinorhodin. Additionally, the Sco2951/Sco5261 mutant shows a decrease in stored triacylglycerides during exponential growth Streptomyces coelicolor
physiological function the enzyme plays a role in antibiotic and triacylglycerol production, e.g. production of the polyketide antibiotic actinorhodin, overview Streptomyces coelicolor