Cloned (Comment) | Organism |
---|---|
gene AtNAD-ME1, DNA and amino acid sequence determination and analysis, phylogenetic tree | Arabidopsis thaliana |
gene AtNAD-ME2, DNA and amino acid sequence determination and analysis, phylogenetic tree | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
NAD+ | pH 7.4, 30°C, isozyme NAD-ME1 | Arabidopsis thaliana | |
0.5 | - |
NAD+ | pH 7.4, 30°C, isozyme NAD-ME2 | Arabidopsis thaliana | |
3 | - |
(S)-malate | pH 7.4, 30°C, isozyme NAD-ME1 | Arabidopsis thaliana | |
3 | - |
(S)-malate | pH 7.4, 30°C, isozyme NAD-ME2 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
2 * 58000, beta-subunit, isozyme NAD-ME2, SDS-PAGE | Arabidopsis thaliana |
65000 | - |
2 * 65000, alpha-subunit, isozyme NAD-ME1, SDS-PAGE | Arabidopsis thaliana |
117500 | - |
gel filtration, isozyme NAD-ME2 | Arabidopsis thaliana |
120000 | - |
gel filtration, isozyme NAD-ME1 | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Arabidopsis thaliana | the enzyme plays a central role in the metabolite flux through the tricarboxylic acid cycle, overview | pyruvate + CO2 + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8L7K9 | isozyme NAD-ME2; isozyme NAD-ME2 encoded by gene AtNAD-ME2 | - |
Arabidopsis thaliana | Q9SIU0 | isozyme NAD-ME1; isozyme NAD-ME1 encoded by gene AtNAD-ME1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | leaf crude extracts contain about 20% higher NAD-ME specific activities at the end of the night period than at the end of the day period, isozyme NAD-ME1 is more abundant during the night period | Arabidopsis thaliana | - |
leaf | leaf crude extracts contain about 20% higher NAD-ME specific activities at the end of the night period than at the end of the day period, isozyme NAD-ME2 is more abundant during the night period | Arabidopsis thaliana | - |
additional information | tissue specific expression of the isozyme, overview | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Arabidopsis thaliana | pyruvate + CO2 + NADH | - |
r | |
(S)-malate + NAD+ | the enzyme plays a central role in the metabolite flux through the tricarboxylic acid cycle, overview | Arabidopsis thaliana | pyruvate + CO2 + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 58000, beta-subunit, isozyme NAD-ME2, SDS-PAGE | Arabidopsis thaliana |
dimer | 2 * 65000, alpha-subunit, isozyme NAD-ME1, SDS-PAGE | Arabidopsis thaliana |
More | the isozyme NAD-ME1 is grouped into the clade that includes enzymes with alpha-subunits with molecular masses of approximately 65 kDa in the plant NAD-ME phylogenetic tree, isozymes NAD-ME1 and NAD-ME2 form both homo- and heterooligomers in vitro and in vivo, overview | Arabidopsis thaliana |
More | the isozyme NAD-ME2 is grouped into the clades with enzymes possessing beta-subunits with molecular masses of approximately 58 kD in the plant NAD-ME phylogenetic tree, isozymes NAD-ME1 and NAD-ME2 form both homo- and heterooligomers in vitro and in vivo, overview | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
NAD-malic enzyme | - |
Arabidopsis thaliana |
NAD-ME1 | - |
Arabidopsis thaliana |
NAD-ME2 | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
31.1 | - |
(S)-malate | pH 7.4, 30°C, isozyme NAD-ME1 | Arabidopsis thaliana | |
44.1 | - |
(S)-malate | pH 7.4, 30°C, isozyme NAD-ME2 | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
- |
Arabidopsis thaliana |
6.6 | - |
- |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arabidopsis thaliana | |
NADH | - |
Arabidopsis thaliana |