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Literature summary for 1.1.1.382 extracted from
- Uncovering rare NADH-preferring ketol-acid reductoisomerases (2014), Metab. Eng., 26C, 17-22.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermacetogenium phaeum |
| expression in Escherichia coli | uncultured archaeon |
| expression in Escherichia coli | Desulfosudis oleivorans |
| expression in Escherichia coli | Archaeoglobus fulgidus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
NADH | Km-value for NADH is below 0.001 mM, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Thermacetogenium phaeum |  |
| 0.005 | - |
NADH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Archaeoglobus fulgidus | |
| 0.032 | - |
NADH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Desulfosudis oleivorans | |
| 1.1 | - |
NADH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | uncultured archaeon | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | O28294 | - |
- |
| Desulfosudis oleivorans | - |
- |
- |
| Thermacetogenium phaeum | - |
- |
- |
| uncultured archaeon | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermacetogenium phaeum |
- |
uncultured archaeon |
- |
Desulfosudis oleivorans |
- |
Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+ | (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate | Thermacetogenium phaeum | (2R)-2,3-dihydroxy-3-methylbutanoate + NAD+ | - |
? | |
| (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+ | (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate | uncultured archaeon | (2R)-2,3-dihydroxy-3-methylbutanoate + NAD+ | - |
? | |
| (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+ | (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate | Desulfosudis oleivorans | (2R)-2,3-dihydroxy-3-methylbutanoate + NAD+ | - |
? | |
| (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+ | (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate | Archaeoglobus fulgidus | (2R)-2,3-dihydroxy-3-methylbutanoate + NAD+ | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
NADH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Archaeoglobus fulgidus | |
| 0.22 | - |
NADH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | uncultured archaeon | |
| 0.25 | - |
NADH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Desulfosudis oleivorans | |
| 0.46 | - |
NADH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Thermacetogenium phaeum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Thermacetogenium phaeum |
| 7 | - |
assay at | uncultured archaeon |
| 7 | - |
assay at | Desulfosudis oleivorans |
| 7 | - |
assay at | Archaeoglobus fulgidus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | specific for NADH. NADH/NADPH ratio of kcat/Km is 13 | Archaeoglobus fulgidus | |
| NADH | specific for NADH. NADH/NADPH ratio of kcat/Km is 152 | uncultured archaeon | |
| NADH | specific for NADH. NADH/NADPH ratio of kcat/Km is 74 | Thermacetogenium phaeum | |
| NADH | specific for NADH. No activity with NADPH | Desulfosudis oleivorans | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
NADH | kcat/Km for NADH is at least 460 /mM*s, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Thermacetogenium phaeum | |
| 8 | - |
NADH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Desulfosudis oleivorans | |
| 20 | - |
NADH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Archaeoglobus fulgidus | |
| 200 | - |
NADH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | uncultured archaeon | |
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