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Literature summary for 1.1.1.381 extracted from
- Characterization of short-chain dehydrogenase/reductase homologues of Escherichia coli (YdfG) and Saccharomyces cerevisiae (YMR226C) (2003), Biochim. Biophys. Acta, 1645, 89-94.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli JM109 | Escherichia coli |
| expression in Escherichia coli JM109 | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.54 | - |
NADP+ | pH 9.0, 30°C | Escherichia coli |  |
| 3 | - |
L-allo-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 7 | - |
D-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 29 | - |
L-allo-threonine | pH 9.0, 30°C | Escherichia coli | |
| 33 | - |
L-Glycerate | pH 9.0, 30°C | Escherichia coli | |
| 36 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 40 | - |
L-serine | pH 9.0, 30°C | Escherichia coli | |
| 50 | - |
D-glycerate | pH 9.0, 30°C | Escherichia coli | |
| 57 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 60 | - |
D-threonine | pH 9.0, 30°C | Escherichia coli | |
| 60 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
| 61 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
| 69 | - |
D-serine | pH 9.0, 30°C | Escherichia coli | |
| 71 | - |
D-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 95 | - |
L-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 95 | - |
L-3-hydroxybutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P39831 | - |
- |
| Saccharomyces cerevisiae | Q05016 | - |
- |
| Saccharomyces cerevisiae ATCC 204508 | Q05016 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.4 | - |
pH 9.0, 30°C | Escherichia coli |
| 6 | - |
pH 9.0, 30°C | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 1% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
| D-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 31% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
| D-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 1% compared to L-allo-threonine | Saccharomyces cerevisiae ATCC 204508 | ? | - |
? | |
| D-glycerate + NADP+ | the Vmax/KM value is 20% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
| D-serine + NADP+ | the Vmax/KM value is 15% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
| D-serine + NADP+ | the Vmax/KM value is less than 1% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
| D-serine + NADP+ | the Vmax/KM value is less than 1% compared to L-allo-threonine | Saccharomyces cerevisiae ATCC 204508 | ? | - |
? | |
| D-threonine + NADP+ | the Vmax/KM value is 55% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
| D-threonine + NADP+ | the Vmax/KM value is 55% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
| D-threonine + NADP+ | the Vmax/KM value is 55% compared to L-allo-threonine | Saccharomyces cerevisiae ATCC 204508 | ? | - |
? | |
| L-3-hydroxybutyrate + NADP+ | the Vmax/KM value is less than 1% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
| L-3-hydroxybutyrate + NADP+ | the Vmax/KM value is less than 1% compared to L-allo-threonine | Saccharomyces cerevisiae ATCC 204508 | ? | - |
? | |
| L-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 3.8% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
| L-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 34% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
| L-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 3.8% compared to L-allo-threonine | Saccharomyces cerevisiae ATCC 204508 | ? | - |
? | |
| L-allo-threonine + NADP+ | highest Vmax/Km value of all substrates tested. The hydroxyl group of L-allo-threonine is oxidized by the enzymes to yield L-2-amino-3-ketobutyrate, which is spontaneously decarboxylated into aminoacetone | Escherichia coli | aminoacetone + CO2 + NADPH + H+ | - |
? | |
| L-allo-threonine + NADP+ | highest Vmax/Km value of all substrates tested. The hydroxyl group of L-allo-threonine is oxidized by the enzymes to yield L-2-amino-3-oxobutyrate, which is spontaneously decarboxylated into aminoacetone | Saccharomyces cerevisiae | aminoacetone + CO2 + NADPH + H+ | - |
? | |
| L-glycerate + NADP+ | the Vmax/KM value is 18% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
| L-serine + NADP+ | the Vmax/KM value is 53% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
| L-serine + NADP+ | the Vmax/KM value is less than 1% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ydfG | - |
Escherichia coli |
| YMR226c | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
| 30 | - |
assay at | Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
when heated for 10 min in 0.1 M potassium phosphate buffer (pH 7.4) containing 0.01% 2-mercaptoethanol and 10% glycerol, the enzyme is stable at up to 40°C | Saccharomyces cerevisiae |
| 55 | - |
when heated for 10 min in 0.1 M potassium phosphate buffer (pH 7.4) containing 0.01% 2-mercaptoethanol and 10% glycerol, the enzyme is stable at up to 55°C | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.17 | - |
L-Glycerate | pH 9.0, 30°C | Escherichia coli | |
| 2.01 | - |
D-glycerate | pH 9.0, 30°C | Escherichia coli | |
| 3.5 | - |
D-serine | pH 9.0, 30°C | Escherichia coli | |
| 3.78 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
| 4.03 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
| 4.2 | - |
L-serine | pH 9.0, 30°C | Escherichia coli | |
| 5.76 | - |
L-allo-threonine | pH 9.0, 30°C | Escherichia coli | |
| 6.55 | - |
D-threonine | pH 9.0, 30°C | Escherichia coli | |
| 7.84 | - |
D-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 8.61 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 9.38 | - |
L-3-hydroxybutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 11 | - |
L-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 20.1 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 43.8 | - |
L-allo-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 52.8 | - |
D-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
oxidation of L-serine | Escherichia coli |
| 8.5 | - |
oxidation of L-serine | Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10 | most stable over the pH-range 6.5 to 10.0 | Escherichia coli |
| 7.5 | 10.5 | most stable over the pH-range 6.5 to 10.0 | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | no activity with NAD+ | Escherichia coli | |
| NADP+ | no activity with NAD+ | Saccharomyces cerevisiae | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.036 | - |
L-Glycerate | pH 9.0, 30°C | Escherichia coli | |
| 0.04 | - |
D-glycerate | pH 9.0, 30°C | Escherichia coli | |
| 0.05 | - |
D-serine | pH 9.0, 30°C | Escherichia coli | |
| 0.062 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
| 0.067 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
| 0.099 | - |
L-3-hydroxybutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 0.105 | - |
L-serine | pH 9.0, 30°C | Escherichia coli | |
| 0.109 | - |
D-threonine | pH 9.0, 30°C | Escherichia coli | |
| 0.11 | - |
D-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 0.116 | - |
L-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 0.151 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 0.199 | - |
L-allo-threonine | pH 9.0, 30°C | Escherichia coli | |
| 0.558 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 7.5 | - |
D-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 14.6 | - |
L-allo-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
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