Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
(S)-malate | 70°C, pH 9.5, cofactor: NADP+ | Methanocaldococcus jannaschii | |
0.15 | - |
(S)-malate | 70°C, pH 9.5, cofactor: NAD+ | Methanocaldococcus jannaschii | |
0.19 | - |
3-sulfopyruvate | 70°C, pH 8.0, cofactor: NADPH | Methanocaldococcus jannaschii | |
0.25 | - |
oxaloacetate | 70°C, pH 8.0, cofactor: NADH | Methanocaldococcus jannaschii | |
0.3 | - |
oxaloacetate | 70°C, pH 8.0, cofactor: NADPH | Methanocaldococcus jannaschii | |
1.3 | - |
3-sulfopyruvate | 70°C, pH 8.0, cofactor: NADH | Methanocaldococcus jannaschii | |
4.6 | - |
(2S)-3-sulfolactate | 70°C, pH 9.5, cofactor: NADP+ | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-sulfopyruvate + NADPH + H+ | Methanocaldococcus jannaschii | - |
(2S)-3-sulfolactate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q60176 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q60176 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-3-sulfolactate + NADP+ | weak activity, Vmax/KM: 1/min*mg. The enzyme does not catalyse the oxidation of (2S)-3-sulfolactate with NAD+ as cofactor | Methanocaldococcus jannaschii | 3-sulfopyruvate + NADPH + H+ | - |
r | |
(S)-malate + NAD+ | Vmax/KM: 4/min*mg. The enzyme prefers NADP+ over NAD+ in oxidation of (S)-malate | Methanocaldococcus jannaschii | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | Vmax/KM: 4/min*mg. The enzyme prefers NADP+ over NAD+ in oxidation of (S)-malate | Methanocaldococcus jannaschii DSM 2661 | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NADP+ | Vmax/KM: 110/min*mg. The enzyme prefers NADP+ over NAD+ in oxidation of (S)-malate | Methanocaldococcus jannaschii | oxaloacetate + NADPH + H+ | - |
r | |
(S)-malate + NADP+ | Vmax/KM: 110/min*mg. The enzyme prefers NADP+ over NAD+ in oxidation of (S)-malate | Methanocaldococcus jannaschii DSM 2661 | oxaloacetate + NADPH + H+ | - |
r | |
3-sulfopyruvate + NADH + H+ | Vmax/KM: 34/min*mg. The enzyme prefers oxaloacetate over 3-sulfopyruvate using NADH as cofactor | Methanocaldococcus jannaschii | (2S)-3-sulfolactate + NAD+ | - |
ir | |
3-sulfopyruvate + NADPH + H+ | - |
Methanocaldococcus jannaschii | (2S)-3-sulfolactate + NADP+ | - |
r | |
3-sulfopyruvate + NADPH + H+ | Vmax/KM: 590/min*mg. The enzyme prefers NADPH over NADH in reduction of 3-sulfopyruvate | Methanocaldococcus jannaschii | (2S)-3-sulfolactate + NADP+ | - |
r | |
additional information | no activity with 2-oxoglutarate and 1-oxo-1,3,4,6-hexanetetracarboxylic acid | Methanocaldococcus jannaschii | ? | - |
? | |
additional information | no activity with 2-oxoglutarate and 1-oxo-1,3,4,6-hexanetetracarboxylic acid | Methanocaldococcus jannaschii DSM 2661 | ? | - |
? | |
oxaloacetate + NADH + H+ | Vmax/KM: 120 /min*mg. The enzyme prefers oxaloacetate over 3-sulfopyruvate using NADH as cofactor. The enzyme prefers NADPH over NADH in reduction of oxaloacetate | Methanocaldococcus jannaschii | (S)-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | Vmax/KM: 120 /min*mg. The enzyme prefers oxaloacetate over 3-sulfopyruvate using NADH as cofactor. The enzyme prefers NADPH over NADH in reduction of oxaloacetate | Methanocaldococcus jannaschii DSM 2661 | (S)-malate + NAD+ | - |
r | |
oxaloacetate + NADPH + H+ | Vmax/KM: 160/min*mg. The enzyme prefers NADPH over NADH in reduction of oxaloacetate | Methanocaldococcus jannaschii | (S)-malate + NADP+ | - |
r | |
oxaloacetate + NADPH + H+ | Vmax/KM: 160/min*mg. The enzyme prefers NADPH over NADH in reduction of oxaloacetate | Methanocaldococcus jannaschii DSM 2661 | (S)-malate + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
MdhII | - |
Methanocaldococcus jannaschii |
MJ0490 | locus name | Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Methanocaldococcus jannaschii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme prefers NADP+ over NAD+ in oxidation of (S)-malate | Methanocaldococcus jannaschii | |
NADH | the enzyme prefers NADPH over NADH in reduction of oxaloacetate | Methanocaldococcus jannaschii | |
NADP+ | the enzyme prefers NADP+ over NAD+ in oxidation of (S)-malate | Methanocaldococcus jannaschii | |
NADPH | the enzyme prefers NADPH over NADH in reduction of oxaloacetate or 3-sulfopyruvate | Methanocaldococcus jannaschii |