Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a C-terminally His6-tagged fusion protein | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
NADPH | pH 7.5, 37°C | Bacillus subtilis | |
0.096 | - |
NADP+ | pH 7.5, 37°C | Bacillus subtilis | |
1.68 | - |
2,4,6/3,5-pentahydroxycyclohexanone | pH 7.5, 37°C | Bacillus subtilis | |
34.3 | - |
scyllo-Inositol | pH 7.5, 37°C | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O32223 | - |
- |
Bacillus subtilis 168 | O32223 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:scyllo-inositol-grown cell | - |
Bacillus subtilis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
scyllo-inositol + NADP+ | no activity with NAD+ | Bacillus subtilis | 2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+ | 2,4,6/3,5-pentahydroxycyclohexanone i.e. (2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone i.e. scyllo-inosose | r | |
scyllo-inositol + NADP+ | no activity with NAD+ | Bacillus subtilis 168 | 2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+ | 2,4,6/3,5-pentahydroxycyclohexanone i.e. (2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone i.e. scyllo-inosose | r |
Synonyms | Comment | Organism |
---|---|---|
IolW | - |
Bacillus subtilis |
yvaA | formerly, gene name | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | no activity with NAD+ | Bacillus subtilis |
Organism | Comment | Expression |
---|---|---|
Bacillus subtilis | induced during growth on scyllo-inositol | up |