Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrobaculum calidifontis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
guanidine hydrochloride | in the presence of 4 M guanidine hydrochloride enzyme structure is unfolded with complete loss of enzyme activity | Pyrobaculum calidifontis | |
additional information | urea cannot induce complete unfolding and inactivation of Pcal_1699 even at a final concentration of 8 M. Not inhibitory: EDTA Triton X-100, TWeen-20, dithiothreitol, glycerol | Pyrobaculum calidifontis | |
sodium dodecylsulfate | 0.1%, 70% loss of activity, 1%, complete loss of activity | Pyrobaculum calidifontis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
oxaloacetate | pH 10, 55°C | Pyrobaculum calidifontis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is not dependent on any metal ions | Pyrobaculum calidifontis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33292 | - |
4 * 33292, calculated, 4 * 35086, MALDI-TOF | Pyrobaculum calidifontis |
35086 | - |
4 * 33292, calculated, 4 * 35086, MALDI-TOF | Pyrobaculum calidifontis |
140000 | - |
gel filtration | Pyrobaculum calidifontis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum calidifontis | A3MWU9 | - |
- |
Pyrobaculum calidifontis JCM 11548 | A3MWU9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADH + H+ | - |
Pyrobaculum calidifontis | (S)-malate + NAD+ | - |
? | |
oxaloacetate + NADH + H+ | - |
Pyrobaculum calidifontis JCM 11548 | (S)-malate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 33292, calculated, 4 * 35086, MALDI-TOF | Pyrobaculum calidifontis |
Synonyms | Comment | Organism |
---|---|---|
Pcal_1699 | - |
Pyrobaculum calidifontis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Pyrobaculum calidifontis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
98 | - |
more than 90% residual activity after 6 h in boiling water. No significant change in CD spectra up to 100°C | Pyrobaculum calidifontis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
77 | - |
oxaloacetate | pH 10, 55°C | Pyrobaculum calidifontis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
carbonate-bicarbonate buffer | Pyrobaculum calidifontis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADPH | Pyrobaculum calidifontis | |
NADH | - |
Pyrobaculum calidifontis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pyrobaculum calidifontis | calculated | - |
6.9 |