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Literature summary for 1.1.1.37 extracted from

  • Madern, D.; Zaccai, G.
    Stabilisation of halophilic malate dehydrogenase from Haloarcula marismortui by divalent cations - effects of temperature, water isotope, cofactor and pH (1997), Eur. J. Biochem., 249, 607-611.
    View publication on PubMed

General Stability

General Stability Organism
at 10 mM MgCl2, residual activity after 24 h in H2O is less than 20% at 4°C, whereas in D2O the protein is stable with 100% residual activity Haloarcula marismortui
at 4°C, the protein at pH 7.0 is stable between 0.5 M and 0.9 M MgCl2. At pH 8.0, it is stable between 0.05 M and 1.2 M MgCl2 Haloarcula marismortui

Organism

Organism UniProt Comment Textmining
Haloarcula marismortui
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxaloacetate + NADH + H+
-
Haloarcula marismortui (S)-malate + NAD+
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Haloarcula marismortui

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-10 35 at MgCl concentrations below 0.5 M or CaCl concentrations below 0.35 M, the stability of the protein increases with decreasing temperature down to -10°C. At higher concentrations (0.8-1.5 M MgCl2 or 0.5-1.0 M CaCl2), the curves are bell-shaped with symmetric decreases in stability around 4°C Haloarcula marismortui

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Haloarcula marismortui