General Stability | Organism |
---|---|
at 10 mM MgCl2, residual activity after 24 h in H2O is less than 20% at 4°C, whereas in D2O the protein is stable with 100% residual activity | Haloarcula marismortui |
at 4°C, the protein at pH 7.0 is stable between 0.5 M and 0.9 M MgCl2. At pH 8.0, it is stable between 0.05 M and 1.2 M MgCl2 | Haloarcula marismortui |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloarcula marismortui | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADH + H+ | - |
Haloarcula marismortui | (S)-malate + NAD+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Haloarcula marismortui |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
-10 | 35 | at MgCl concentrations below 0.5 M or CaCl concentrations below 0.35 M, the stability of the protein increases with decreasing temperature down to -10°C. At higher concentrations (0.8-1.5 M MgCl2 or 0.5-1.0 M CaCl2), the curves are bell-shaped with symmetric decreases in stability around 4°C | Haloarcula marismortui |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Haloarcula marismortui |