Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Haloarcula marismortui |
Protein Variants | Comment | Organism |
---|---|---|
E243R | mutation does not affect enzyme activity. The mutant enzyme is more halophilic than the wild-type protein. It is more sensitive to temperature and requires significantly higher concentrations of NaCl or KCl for equivalent stability | Haloarcula marismortui |
General Stability | Organism |
---|---|
in KCl at 25°C, 50% residual activity is observed after 24 h for mutant enzyme E243R in 2.3 M salt, compared to 1.3 M salt for wild-type enzyme. In KCl at 4°C, the mutant shows 50% stability in 1.1 M salt | Haloarcula marismortui |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloarcula marismortui | Q07841 | - |
- |
Haloarcula marismortui DSM 3752 | Q07841 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloarcula marismortui |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADH + H+ | - |
Haloarcula marismortui | (S)-malate + NAD+ | - |
? | |
oxaloacetate + NADH + H+ | - |
Haloarcula marismortui DSM 3752 | (S)-malate + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MalDH | - |
Haloarcula marismortui |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Haloarcula marismortui |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
63 | - |
t1/2 is 510 min for the wild-type enzyme and 15 min for mutant enzyme E243R | Haloarcula marismortui |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Haloarcula marismortui |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Haloarcula marismortui |