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Literature summary for 1.1.1.37 extracted from

  • Cendrin, F.; Chroboczek, J.; Zaccai, G.; Eisenberg, H.; Mevarech, M.
    Cloning, sequencing, and expression in Escherichia coli of the gene coding for malate dehydrogenase of the extremely halophilic archaebacterium Haloarcula marismortui (1993), Biochemistry, 32, 4308-4313.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned with the Escherichia coli expression vector pET11a, large amounts of a soluble but inactive form of the enzyme are produced upon its induction Haloarcula marismortui

Protein Variants

Protein Variants Comment Organism
R100Q the mutant enzyme has considerably higher specificity for pyruvate than for oxaloacetate. Whereas the Km value for pyruvate is increased about 2fold, that for oxaloacetate increases 30fold. The R100Q mutant is not subjected to substrate inhibition, at least not at substrate concentrations up to 30 mM, and the highest kcat value is obtained at the lowest salt concentration used (0.15 M NaCl) Haloarcula marismortui

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
oxaloacetate pH 7.0, temperature not specified in the publication, 4 M NaCl, wild-type enzyme Haloarcula marismortui
0.9
-
oxaloacetate pH 7.0, temperature not specified in the publication, 2 M NaCl, wild-type enzyme Haloarcula marismortui
2.2
-
pyruvate pH 7.0, temperature not specified in the publication, 0.15 M NaCl, mutant enzyme R100Q Haloarcula marismortui
2.2
-
pyruvate pH 7.0, temperature not specified in the publication, 2 M NaCl, mutant enzyme R100Q Haloarcula marismortui
2.9
-
pyruvate pH 7.0, temperature not specified in the publication, 4 M NaCl, mutant enzyme R100Q Haloarcula marismortui
21.8
-
oxaloacetate pH 7.0, temperature not specified in the publication, 4 M NaCl, mutant enzyme R100Q Haloarcula marismortui
29.4
-
oxaloacetate pH 7.0, temperature not specified in the publication, 2 M NaCl, mutant enzyme R100Q Haloarcula marismortui

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl activation of the enzyme was obtained by increasing the salt concentration to 3 M NaCl Haloarcula marismortui

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32638
-
x * 32638, calculated from sequence Haloarcula marismortui

Organism

Organism UniProt Comment Textmining
Haloarcula marismortui Q07841
-
-
Haloarcula marismortui DSM 3752 Q07841
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Haloarcula marismortui

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxaloacetate + NADH + H+
-
Haloarcula marismortui (S)-malate + NAD+
-
?
oxaloacetate + NADH + H+
-
Haloarcula marismortui DSM 3752 (S)-malate + NAD+
-
?
pyruvate + NADH + H+ wild-type enzyme shows no activity with pyruvate. Mutant enzyme R100Q has considerably higher specificity for pyruvate than for oxaloacetate Haloarcula marismortui (S)-lactate + NAD+
-
?
pyruvate + NADH + H+ wild-type enzyme shows no activity with pyruvate. Mutant enzyme R100Q has considerably higher specificity for pyruvate than for oxaloacetate Haloarcula marismortui DSM 3752 (S)-lactate + NAD+
-
?

Subunits

Subunits Comment Organism
? x * 32638, calculated from sequence Haloarcula marismortui

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
oxaloacetate pH 7.0, temperature not specified in the publication, 4 M NaCl, mutant enzyme R100Q Haloarcula marismortui
2
-
oxaloacetate pH 7.0, temperature not specified in the publication, 2 M NaCl, mutant enzyme R100Q Haloarcula marismortui
2.1
-
pyruvate pH 7.0, temperature not specified in the publication, 2 M NaCl, mutant enzyme R100Q Haloarcula marismortui
3.5
-
pyruvate pH 7.0, temperature not specified in the publication, 4 M NaCl, mutant enzyme R100Q Haloarcula marismortui
6 9 oxaloacetate pH 7.0, temperature not specified in the publication, 4 M NaCl, wild-type enzyme Haloarcula marismortui
12.3
-
pyruvate pH 7.0, temperature not specified in the publication, 0.15 M NaCl, mutant enzyme R100Q Haloarcula marismortui
197
-
oxaloacetate pH 7.0, temperature not specified in the publication, 2 M NaCl, wild-type enzyme Haloarcula marismortui

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Haloarcula marismortui

Cofactor

Cofactor Comment Organism Structure
NADH
-
Haloarcula marismortui

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0014
-
oxaloacetate pH 7.0, temperature not specified in the publication, 4 M NaCl, mutant enzyme R100Q Haloarcula marismortui
0.007
-
oxaloacetate pH 7.0, temperature not specified in the publication, 2 M NaCl, mutant enzyme R100Q Haloarcula marismortui
1
-
pyruvate pH 7.0, temperature not specified in the publication, 2 M NaCl, mutant enzyme R100Q Haloarcula marismortui
1.2
-
pyruvate pH 7.0, temperature not specified in the publication, 4 M NaCl, mutant enzyme R100Q Haloarcula marismortui
5.6
-
pyruvate pH 7.0, temperature not specified in the publication, 0.15 M NaCl, mutant enzyme R100Q Haloarcula marismortui
100
-
oxaloacetate pH 7.0, temperature not specified in the publication, 4 M NaCl, wild-type enzyme Haloarcula marismortui
200
-
oxaloacetate pH 7.0, temperature not specified in the publication, 2 M NaCl, wild-type enzyme Haloarcula marismortui