Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.37 extracted from

  • Ge, Y.D.; Cao, Z.Y.; Wang, Z.D.; Chen, L.L.; Zhu, Y.M.; Zhu, G.P.
    Identification and biochemical characterization of a thermostable malate dehydrogenase from the mesophile Streptomyces coelicolor A3(2) (2010), Biosci. Biotechnol. Biochem., 74, 2194-2201.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information MDH is not affected by DTT or EDTA Streptomyces coelicolor

Cloned(Commentary)

Cloned (Comment) Organism
gene mdh, sequence comparisons and phylogenetic analysis, overview, overexpression of His6-tagged MDH in Escherichia coli strain Rosetta(DE3), subcloning in Escherichia coli strain DH5alpha Streptomyces coelicolor

Inhibitors

Inhibitors Comment Organism Structure
(S)-malate substrate inhibition, 60% inhibition at 30 mM Streptomyces coelicolor
Co2+ 36% inhibition at 2 mM Streptomyces coelicolor
DMSO
-
Streptomyces coelicolor
Fe2+ complete inhibition at 2 mM Streptomyces coelicolor
additional information MDH is not affected by DTT or EDTA Streptomyces coelicolor
oxaloacetate substrate inhibition, 50% inhibition at 6 mM Streptomyces coelicolor
Triton X-100
-
Streptomyces coelicolor
Zn2+ 73% inhibition at 2 mM Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
NAD+ pH 8.5, 30°C Streptomyces coelicolor
0.189
-
oxaloacetate pH 8.5, 30°C Streptomyces coelicolor
0.494
-
(S)-malate pH 8.5, 30°C Streptomyces coelicolor
0.83
-
NADH pH 8.5, 30°C Streptomyces coelicolor

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cytosolic MDH Streptomyces coelicolor 5829
-
mitochondrion mitochondrial MDH Streptomyces coelicolor 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information enzyme activity is not or poorly affected by 2 mM of Ca2+, Mn2+, Cu2+, Mg2+, Na+, Li+, K+, Rb+, and Ag+ Streptomyces coelicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36675
-
2 * 36675, MALDI-TOF mass spectrometry Streptomyces coelicolor
36675
-
4 * 36675, MALDI-TOF mass spectrometry Streptomyces coelicolor
73350
-
dimeric enzyme, MALDI-TOF mass spectrometry Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Streptomyces coelicolor
-
oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+ Streptomyces coelicolor A3(2)
-
oxaloacetate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor Q9K3J3 gene mdh
-
Streptomyces coelicolor A3(2) Q9K3J3 gene mdh
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged MDH from Escherichia coli strain Rosetta(DE3) by cobalt affinity chromatography Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Streptomyces coelicolor oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+ the reverse reaction is highly preferred, with a 1050fold higher kcat/Km value Streptomyces coelicolor oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Streptomyces coelicolor A3(2) oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+ the reverse reaction is highly preferred, with a 1050fold higher kcat/Km value Streptomyces coelicolor A3(2) oxaloacetate + NADH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 36675, MALDI-TOF mass spectrometry Streptomyces coelicolor
More structure analysis and comparison, MALDI-TOF mass spectrometry, overview Streptomyces coelicolor
tetramer 4 * 36675, MALDI-TOF mass spectrometry Streptomyces coelicolor

Synonyms

Synonyms Comment Organism
(S)-malate dehydrogenase
-
Streptomyces coelicolor
More MDH belongs to the superfamily of 2-ketoacid NAD(P)+-dependent dehydrogenases Streptomyces coelicolor
NAD+-dependent MDH
-
Streptomyces coelicolor
NAD-MDH
-
Streptomyces coelicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
reduction and oxidation Streptomyces coelicolor

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60 profile, overview Streptomyces coelicolor

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
20 min, purified recombinant enzyme, 82% activity remaining Streptomyces coelicolor
50
-
purified recombinant enzyme, 70% activity remaining after 20 min, 71% after 30 min, 60% after 90 min, and 50% after 120 min Streptomyces coelicolor
55
-
20 min, purified recombinant enzyme, 10% activity remaining Streptomyces coelicolor
60
-
20 min, purified recombinant enzyme, complete inactivation Streptomyces coelicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.66
-
NAD+ pH 8.5, 30°C Streptomyces coelicolor
4.71
-
(S)-malate pH 8.5, 30°C Streptomyces coelicolor
542
-
NADH pH 8.5, 30°C Streptomyces coelicolor
1870
-
oxaloacetate pH 8.5, 30°C Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
50°C, phosphate buffer, recombinant enzyme, reduction and oxidation Streptomyces coelicolor
8.5
-
30°C, Tris-HCl buffer, recombinant enzyme, reduction and oxidation Streptomyces coelicolor

pH Range

pH Minimum pH Maximum Comment Organism
5.7 10 profiles in different buffers, overview Streptomyces coelicolor

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on and specific for Streptomyces coelicolor
NADH dependent on and specific for Streptomyces coelicolor

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.8
-
oxaloacetate pH 8.5, 30°C Streptomyces coelicolor
12.8
-
(S)-malate pH 8.5, 30°C Streptomyces coelicolor

General Information

General Information Comment Organism
physiological function mitochondrial and cytosolic MDH isozymes are required for maintaining the balance of NAD+ and NADH in mitochondria and cytosol Streptomyces coelicolor

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9.53
-
(S)-malate pH 8.5, 30°C Streptomyces coelicolor
24.4
-
NAD+ pH 8.5, 30°C Streptomyces coelicolor
6560
-
NADH pH 8.5, 30°C Streptomyces coelicolor
10000
-
oxaloacetate pH 8.5, 30°C Streptomyces coelicolor