Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Haloarcula marismortui |
Protein Variants | Comment | Organism |
---|---|---|
K205A | site-directed mutagenesis, the oligomeric state of the mutant changes with the nature of the anion bound, the mutant is dimeric or tetrameric, overview | Haloarcula marismortui |
R207S/R292S | site-directed mutagenesis, the oligomeric state of the mutant changes with the nature of the anion bound, the mutant is dimeric or tetrameric, overview | Haloarcula marismortui |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Haloarcula marismortui | |
0.012 | - |
NADH | pH 8.0, 25°C, recombinant wild-type enzyme and mutant K205A | Haloarcula marismortui | |
0.13 | - |
oxaloacetate | pH 8.0, 25°C, recombinant wild-type enzyme and mutant K205A | Haloarcula marismortui |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
chloride | binding site structure analysis, structural adaptation in the halophilic protein, residue Lys205 is involved, the anion influences the oligomeric state of the enzyme, overview | Haloarcula marismortui |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Haloarcula marismortui | - |
oxaloacetate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloarcula marismortui | Q07841 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by gel filtration, hydrophobic interaction chromatography using 4 M KF and 1.3 M ammonium sulfate for elution, followed by dialysis | Haloarcula marismortui |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Haloarcula marismortui | oxaloacetate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Haloarcula marismortui |
Synonyms | Comment | Organism |
---|---|---|
halophilic malate dehydrogenase | - |
Haloarcula marismortui |
MalDH | - |
Haloarcula marismortui |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at, reduction reaction | Haloarcula marismortui |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at, reduction reaction | Haloarcula marismortui |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Haloarcula marismortui | |
NADH | - |
Haloarcula marismortui |