Cloned (Comment) | Organism |
---|---|
mitochondrial and glycosomal malate dehydrogenase isoenzyme, functional expression in Escherichia coli | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
oxaloacetate | glycosomal enzyme | Trypanosoma brucei | |
0.036 | - |
NADH | glycosomal enzyme | Trypanosoma brucei | |
0.054 | - |
NADH | pH 7.5, 37°C, glycosomal enzyme | Trypanosoma brucei | |
0.069 | - |
oxaloacetate | glycosomal enzyme | Trypanosoma brucei | |
0.085 | - |
NADH | pH 7.5, 37°C, glycosomal enzyme | Trypanosoma brucei | |
0.32 | - |
NADH | glacosomal enzyme | Trypanosoma brucei | |
0.56 | - |
(S)-malate | pH 7.5, 37°C, mitochondrial enzyme | Trypanosoma brucei | |
2.7 | - |
p-hydroxyphenylpyruvate | - |
Trypanosoma brucei | |
2.72 | - |
(S)-malate | pH 7.5, 37°C, glycosomal enzyme | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | cytosolic isoenzyme is the only isoform of malate dehydrogenase expressed in the bloodstream form | Trypanosoma brucei | 5829 | - |
glycosome | - |
Trypanosoma brucei | 20015 | - |
mitochondrion | - |
Trypanosoma brucei | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Trypanosoma brucei | mitochondrial enzyme, Krebs cycle | oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH | Trypanosoma brucei | cytosolic enzyme and glycosomal enzyme | (S)-malate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | O15769 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bloodstream form | the cytosolic isoenzyme is the only isoform of malate dehydrogenase expressed in the bloodstream form | Trypanosoma brucei | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | mitochondrial enzyme, Krebs cycle | Trypanosoma brucei | oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH | cytosolic enzyme and glycosomal enzyme | Trypanosoma brucei | (S)-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Trypanosoma brucei | (S)-malate + NAD+ | - |
r | |
p-hydroxyphenylpyruvate + NADH + H+ | - |
Trypanosoma brucei | 2-hydroxy-3-(4-hydroxyphenyl)propanoate | - |
? | |
phenylpyruvate + NADH + H+ | - |
Trypanosoma brucei | 2-hydroxy-3-phenylpropanoate + NAD+ | - |
? |