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Literature summary for 1.1.1.37 extracted from

  • Tripodi, K.E.J.; Gomez Casati, M.E.; Uttaro, A.D.; Podesta, F.E.
    Expression and characterization of recombinant cytosolic NAD+-dependent malate dehydrogenase from Mesembryanthemum crystallinum (2003), Physiol. Plant., 117, 222-228.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mesembryanthemum crystallinum

Inhibitors

Inhibitors Comment Organism Structure
oxaloacetate
-
Mesembryanthemum crystallinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0088
-
oxaloacetate pH 6.8, 30°C Mesembryanthemum crystallinum
0.0136
-
NADH pH 8.2, 30°C Mesembryanthemum crystallinum
0.0341
-
L-malate pH 8.2, 30°C Mesembryanthemum crystallinum
0.0366
-
NADH pH 6.8, 30°C Mesembryanthemum crystallinum
0.0449
-
oxaloacetate pH 8.2, 30°C Mesembryanthemum crystallinum
0.319
-
NAD+ pH 8.2, 30°C Mesembryanthemum crystallinum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Mesembryanthemum crystallinum 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39500
-
x * 39500, SDS-PAGE Mesembryanthemum crystallinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mesembryanthemum crystallinum the enzyme plays a central role in Crassulacean acid metabolism ?
-
?

Organism

Organism UniProt Comment Textmining
Mesembryanthemum crystallinum O24047
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Mesembryanthemum crystallinum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NADH + H+ about 10% of the activity with oxaloacetate at pH 6.8 and at pH 8.2 Mesembryanthemum crystallinum 2-hydroxyglutarate + NAD+
-
?
3-bromopyruvate + NADH about 10% of the activity with oxaloacetate at pH 6.8 Mesembryanthemum crystallinum 3-bromo-2-hydroxypropanoate + NAD+
-
?
alpha-ketoisovalerate + NADH about 10% of the activity with oxaloacetate at pH 8.2 Mesembryanthemum crystallinum 2-hydroxyisovalerate + NAD+
-
?
additional information the enzyme plays a central role in Crassulacean acid metabolism Mesembryanthemum crystallinum ?
-
?
additional information less than 10% of the activity with oxaloacetate with the substrates: alpha-ketobutyrate, alpha-ketoisocaproate, alpha-ketovalerate and alpha-ketomethylvalerate Mesembryanthemum crystallinum ?
-
?
oxaloacetate + NADH the ratio of oxaloacetate reduction to malate oxidation is 28.4 Mesembryanthemum crystallinum L-malate + NAD+
-
r
phenylpyruvate + NADH about 30% of the activity with oxaloacetate at pH 8.2 Mesembryanthemum crystallinum 2-hydroxy-3-phenylpropanoate + NAD+
-
?
pyruvate + NADH about 25% of the activity with oxaloacetate at pH 8.2 Mesembryanthemum crystallinum 2-hydroxypropanoate + NAD+
-
?

Subunits

Subunits Comment Organism
? x * 39500, SDS-PAGE Mesembryanthemum crystallinum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
reduction of oxaloacetate Mesembryanthemum crystallinum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 58 30°C: about 50% of maximal activity, 58°C: about 55% of maximal activity Mesembryanthemum crystallinum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
5 min, stable up to Mesembryanthemum crystallinum
60
-
5 min, about 80% loss of activity Mesembryanthemum crystallinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9.5 reduction of oxaloacetate Mesembryanthemum crystallinum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Mesembryanthemum crystallinum
NADH
-
Mesembryanthemum crystallinum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.225
-
oxaloacetate pH 6.8, 30°C Mesembryanthemum crystallinum
0.775
-
oxaloacetate pH 8.2, 30°C Mesembryanthemum crystallinum