Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mesembryanthemum crystallinum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
oxaloacetate | - |
Mesembryanthemum crystallinum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0088 | - |
oxaloacetate | pH 6.8, 30°C | Mesembryanthemum crystallinum | |
0.0136 | - |
NADH | pH 8.2, 30°C | Mesembryanthemum crystallinum | |
0.0341 | - |
L-malate | pH 8.2, 30°C | Mesembryanthemum crystallinum | |
0.0366 | - |
NADH | pH 6.8, 30°C | Mesembryanthemum crystallinum | |
0.0449 | - |
oxaloacetate | pH 8.2, 30°C | Mesembryanthemum crystallinum | |
0.319 | - |
NAD+ | pH 8.2, 30°C | Mesembryanthemum crystallinum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mesembryanthemum crystallinum | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39500 | - |
x * 39500, SDS-PAGE | Mesembryanthemum crystallinum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mesembryanthemum crystallinum | the enzyme plays a central role in Crassulacean acid metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesembryanthemum crystallinum | O24047 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Mesembryanthemum crystallinum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NADH + H+ | about 10% of the activity with oxaloacetate at pH 6.8 and at pH 8.2 | Mesembryanthemum crystallinum | 2-hydroxyglutarate + NAD+ | - |
? | |
3-bromopyruvate + NADH | about 10% of the activity with oxaloacetate at pH 6.8 | Mesembryanthemum crystallinum | 3-bromo-2-hydroxypropanoate + NAD+ | - |
? | |
alpha-ketoisovalerate + NADH | about 10% of the activity with oxaloacetate at pH 8.2 | Mesembryanthemum crystallinum | 2-hydroxyisovalerate + NAD+ | - |
? | |
additional information | the enzyme plays a central role in Crassulacean acid metabolism | Mesembryanthemum crystallinum | ? | - |
? | |
additional information | less than 10% of the activity with oxaloacetate with the substrates: alpha-ketobutyrate, alpha-ketoisocaproate, alpha-ketovalerate and alpha-ketomethylvalerate | Mesembryanthemum crystallinum | ? | - |
? | |
oxaloacetate + NADH | the ratio of oxaloacetate reduction to malate oxidation is 28.4 | Mesembryanthemum crystallinum | L-malate + NAD+ | - |
r | |
phenylpyruvate + NADH | about 30% of the activity with oxaloacetate at pH 8.2 | Mesembryanthemum crystallinum | 2-hydroxy-3-phenylpropanoate + NAD+ | - |
? | |
pyruvate + NADH | about 25% of the activity with oxaloacetate at pH 8.2 | Mesembryanthemum crystallinum | 2-hydroxypropanoate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39500, SDS-PAGE | Mesembryanthemum crystallinum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
reduction of oxaloacetate | Mesembryanthemum crystallinum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 58 | 30°C: about 50% of maximal activity, 58°C: about 55% of maximal activity | Mesembryanthemum crystallinum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
5 min, stable up to | Mesembryanthemum crystallinum |
60 | - |
5 min, about 80% loss of activity | Mesembryanthemum crystallinum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | reduction of oxaloacetate | Mesembryanthemum crystallinum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mesembryanthemum crystallinum | |
NADH | - |
Mesembryanthemum crystallinum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.225 | - |
oxaloacetate | pH 6.8, 30°C | Mesembryanthemum crystallinum | |
0.775 | - |
oxaloacetate | pH 8.2, 30°C | Mesembryanthemum crystallinum |