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Literature summary for 1.1.1.37 extracted from

  • Aguero, F.; Noe, G.; Hellman, U.; Repetto, Y.; Zaha, A.; Cazzulo, J.J.
    Purification, cloning, and expression of the mitochondrial malate dehydrogenase (mMDH) from protoscolices of Echinococcus granulosus (2004), Mol. Biochem. Parasitol., 137, 207-214.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as protein fusion with glutathione S-transferase or a poly-His tail Echinococcus granulosus

Inhibitors

Inhibitors Comment Organism Structure
additional information unaffected by excess of L-malate Echinococcus granulosus
oxaloacetate above 0.025 mM Echinococcus granulosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
oxaloacetate pH 8.0, native enzyme Echinococcus granulosus
0.007
-
NADH pH 8.0, native enzyme Echinococcus granulosus
0.0127
-
NADH pH 8.0, recombinant enzyme with a poly-His tai Echinococcus granulosus
0.013
-
oxaloacetate pH 8.0, recombinant protein fusions with glutathione S-transferase Echinococcus granulosus
0.018
-
oxaloacetate pH 8.0, recombinant enzyme with a poly-His tail Echinococcus granulosus
0.019
-
NADH pH 8.0, recombinant protein fusions with glutathione S-transferase Echinococcus granulosus
0.027
-
NAD+ pH 7.6, native enzyme Echinococcus granulosus
0.033
-
L-malate pH 7.6, native enzyme Echinococcus granulosus
0.033
-
NAD+ pH 7.6, recombinant enzyme with a poly-His tail Echinococcus granulosus
0.036
-
NAD+ pH 7.6, recombinant protein fusions with glutathione S-transferase Echinococcus granulosus
0.047
-
L-malate pH 7.6, recombinant enzyme with a poly-His tail Echinococcus granulosus
0.054
-
L-malate pH 7.6, native enzyme, recombinant protein fusions with glutathione S-transferase Echinococcus granulosus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Echinococcus granulosus 5739
-

Organism

Organism UniProt Comment Textmining
Echinococcus granulosus Q704F5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Echinococcus granulosus

Source Tissue

Source Tissue Comment Organism Textmining
protoscolex
-
Echinococcus granulosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
238.1
-
-
Echinococcus granulosus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Echinococcus granulosus
NADH
-
Echinococcus granulosus