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Literature summary for 1.1.1.365 extracted from

  • Kuorelahti, S.; Kalkkinen, N.; Penttilä, M.; Londesborough, J.; Richard, P.
    Identification in the mold Hypocrea jecorina of the first fungal D-galacturonic acid reductase (2005), Biochemistry, 44, 11234-11240.
    View publication on PubMed
Search for more literature for 1.1.1.365

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain CEN.PK2-1B Trichoderma reesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 NADP+ in 100 mM sodium phosphate (pH 7.0), at 22°C Trichoderma reesei
0.03
-
 NADPH in 100 mM sodium phosphate (pH 7.0), at 22°C Trichoderma reesei
4
-
 L-galactonate in 100 mM sodium phosphate (pH 7.0), at 22°C Trichoderma reesei
6
-
 DL-glyceraldehyde in 100 mM sodium phosphate (pH 7.0), at 22°C Trichoderma reesei
6
-
 D-galacturonate in 100 mM sodium phosphate (pH 7.0), at 22°C Trichoderma reesei
11
-
 D-glucuronic acid in 100 mM sodium phosphate (pH 7.0), at 22°C Trichoderma reesei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, SDS-PAGE Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Trichoderma reesei
-
-
-
Trichoderma reesei RUT C-30
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Fractogel TSK DEAE-650 column chromatography Trichoderma reesei

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:D-galacturonate-grown cell
-
 Trichoderma reesei
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galacturonate + NADPH + H+
-
 Trichoderma reesei L-galactonate + NADP+
-
 r
D-galacturonate + NADPH + H+
-
 Trichoderma reesei RUT C-30 L-galactonate + NADP+
-
 r
D-glucuronic acid + NADPH + H+
-
 Trichoderma reesei ?
-
 ?
D-glucuronic acid + NADPH + H+
-
 Trichoderma reesei RUT C-30 ?
-
 ?
DL-glyceraldehyde + NADPH + H+
-
 Trichoderma reesei ?
-
 ?
DL-glyceraldehyde + NADPH + H+
-
 Trichoderma reesei RUT C-30 ?
-
 ?
L-galactonate + NADP+
-
 Trichoderma reesei D-galacturonate + NADPH + H+
-
 r
L-galactonate + NADP+
-
 Trichoderma reesei RUT C-30 D-galacturonate + NADPH + H+
-
 r
additional information no activity with NADH and D-glucose, D-fructose, D-xylose, D-galactose, L-arabinose, or D-mannose. No backward reaction with L-gulonic acid, glycerol, D-arabitol, L-arabitol, xylitol, galactitol, or ribitol Trichoderma reesei ?
-
 ?
additional information no activity with NADH and D-glucose, D-fructose, D-xylose, D-galactose, L-arabinose, or D-mannose. No backward reaction with L-gulonic acid, glycerol, D-arabitol, L-arabitol, xylitol, galactitol, or ribitol Trichoderma reesei RUT C-30 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Trichoderma reesei

Synonyms

Synonyms Comment Organism
D-galacturonic acid reductase
-
 Trichoderma reesei

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on Trichoderma reesei