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Literature summary for 1.1.1.36 extracted from

  • Belova, L.L.; Sokolov, A.P.; Sidorov, I.A.; Trotsenko, Y.A.
    Purification and characterization of NADPH-dependent acetoacetyl-CoA reductase from Methylobacterium extorquens (1997), FEMS Microbiol. Lett., 156, 275-279.
No PubMed abstract available
Search for more literature for 1.1.1.36

Activating Compound

Activating Compound Comment Organism Structure
alpha-ketoglutarate 20% activation Methylorubrum extorquens
ATP 32% activation Methylorubrum extorquens
citrate 30% activation Methylorubrum extorquens
isocitrate 25% activation, stimulates NADP+ regeneration Methylorubrum extorquens

Inhibitors

Inhibitors Comment Organism Structure
acetoacetyl-CoA
-
 Azotobacter vinelandii
acetoacetyl-CoA
-
 Cupriavidus necator
acetoacetyl-CoA concentration above 40 mM Methylorubrum extorquens
acetoacetyl-CoA
-
 Methylorubrum rhodesianum
acetoacetyl-CoA
-
 Zoogloea ramigera
NAD+ 17% inhibition Methylorubrum extorquens
NAD+
-
 Methylorubrum rhodesianum
NADP+ 40% inhibition Methylorubrum extorquens
NADP+
-
 Methylorubrum rhodesianum
NADPH
-
 Azotobacter vinelandii
NADPH concentrationgreater 100 mM Methylorubrum extorquens
NADPH
-
 Methylorubrum rhodesianum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 acetoacetyl-CoA
-
 Cupriavidus necator
0.011
-
 acetoacetyl-CoA 100 mM NADPH Azotobacter vinelandii
0.0116
-
 acetoacetyl-CoA
-
 Methylorubrum extorquens
0.015
-
 acetoacetyl-CoA
-
 Methylorubrum rhodesianum
0.018
-
 NADPH
-
 Methylorubrum rhodesianum
0.019
-
 NADPH
-
 Cupriavidus necator
0.02
-
 NADPH 32 mM acetoacetyl-CoA Azotobacter vinelandii
0.03
-
 D-beta-hydroxybutyryl-CoA
-
 Methylorubrum rhodesianum
0.031
-
 NADP+
-
 Cupriavidus necator
0.033
-
 D-hydroxybutyryl-CoA
-
 Cupriavidus necator
0.041
-
 NADPH
-
 Methylorubrum extorquens
0.06
-
 NADP+
-
 Methylorubrum rhodesianum
0.25
-
 NAD+
-
 Cupriavidus necator
0.4
-
 NADH
-
 Cupriavidus necator

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Zoogloea ramigera
-
-
soluble
-
 Methylorubrum extorquens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 4 * 31000, 12% PAGE Methylorubrum extorquens
77000
-
-
 Azotobacter vinelandii
84000
-
-
 Cupriavidus necator
92000
-
-
 Zoogloea ramigera
140000
-
 gel-filtration Methylorubrum extorquens
141000
-
 gradient PAGE Methylorubrum extorquens
250000
-
-
 Methylorubrum rhodesianum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetoacetyl-CoA + NADH + H+ Zoogloea ramigera
-
 L-3-hydroxybutyryl-CoA + NAD+
-
 ?
acetoacetyl-CoA + NADH + H+ Rhodospirillum rubrum
-
 L-3-hydroxybutyryl-CoA + NAD+
-
 ?
acetoacetyl-CoA + NADPH + H+ Zoogloea ramigera PHB synthesis D-3-hydroxybutyryl-CoA + NADP+
-
 r
acetoacetyl-CoA + NADPH + H+ Cupriavidus necator PHB synthesis D-3-hydroxybutyryl-CoA + NADP+
-
 ?
acetoacetyl-CoA + NADPH + H+ Methylorubrum extorquens tricarboxylic acid cycle, poly-3-hydroxybutyrate synthesis, aerobic serine pathway D-3-hydroxybutyryl-CoA + NADP+
-
 r
acetoacetyl-CoA + NADPH + H+ Methylorubrum extorquens 15 tricarboxylic acid cycle, poly-3-hydroxybutyrate synthesis, aerobic serine pathway D-3-hydroxybutyryl-CoA + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-
Cupriavidus necator
-
-
-
Methylorubrum extorquens
-
 15
-
Methylorubrum extorquens 15
-
 15
-
Methylorubrum rhodesianum
-
 MB 126
-
Methylorubrum rhodesianum MB 126
-
 MB 126
-
Rhodospirillum rubrum
-
-
-
Zoogloea ramigera
-
-
-

Purification (Commentary)

Purification (Comment) Organism
PEG, DEAE-Toyopearl column Methylorubrum extorquens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
184
-
-
 Methylorubrum extorquens

Storage Stability

Storage Stability Organism
0°C, 25 mM Tris-HCl, pH 8.0 Methylorubrum extorquens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetoacetyl-CoA + NADH + H+
-
 Zoogloea ramigera L-3-hydroxybutyryl-CoA + NAD+
-
 ?
acetoacetyl-CoA + NADH + H+
-
 Cupriavidus necator L-3-hydroxybutyryl-CoA + NAD+
-
 ?
acetoacetyl-CoA + NADH + H+
-
 Rhodospirillum rubrum L-3-hydroxybutyryl-CoA + NAD+
-
 ?
acetoacetyl-CoA + NADPH + H+
-
 Methylorubrum rhodesianum D-beta-hydroxybutyryl-CoA + NADP+
-
 ?
acetoacetyl-CoA + NADPH + H+
-
 Methylorubrum rhodesianum MB 126 D-beta-hydroxybutyryl-CoA + NADP+
-
 ?
acetoacetyl-CoA + NADPH + H+ PHB synthesis Zoogloea ramigera D-3-hydroxybutyryl-CoA + NADP+
-
 r
acetoacetyl-CoA + NADPH + H+ PHB synthesis Cupriavidus necator D-3-hydroxybutyryl-CoA + NADP+
-
 ?
acetoacetyl-CoA + NADPH + H+ tricarboxylic acid cycle, poly-3-hydroxybutyrate synthesis, aerobic serine pathway Methylorubrum extorquens D-3-hydroxybutyryl-CoA + NADP+
-
 r
acetoacetyl-CoA + NADPH + H+ tricarboxylic acid cycle, poly-3-hydroxybutyrate synthesis, aerobic serine pathway Methylorubrum extorquens 15 D-3-hydroxybutyryl-CoA + NADP+
-
 r
acetoacetyl-CoA + NADPH + H+
-
 Azotobacter vinelandii (R)-3-hydroxybutanoyl-CoA +NADP+
-
 r

Subunits

Subunits Comment Organism
tetramer
-
 Zoogloea ramigera
tetramer 4 * 31000, 12% PAGE Methylorubrum extorquens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Zoogloea ramigera
30
-
-
 Methylorubrum extorquens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Methylorubrum extorquens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Azotobacter vinelandii
NADPH
-
 Cupriavidus necator
NADPH
-
 Methylorubrum rhodesianum
NADPH absolute specificity Zoogloea ramigera
NADPH absolute specificity Methylorubrum extorquens