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Literature summary for 1.1.1.359 extracted from

  • Giardina, P.; DeBasia, M.G.; DeRosa, M.; Gambacort, A.; Buonocore, V.
    Glucose dehydrogenase from the thermoacidophilic archaebacterium Sulfolobus solfataricus (1986), Biochem. J., 239, 517-522.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.359

General Stability

General Stability Organism
dehydrogenase preparations became inactivated irreversibly when stored in the absence of ethylene glycol and Mg2+, at very low protein concentration, or during freezing and thawing Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 1 mM, pH 9, 22°C, rapid inactivation Saccharolobus solfataricus
5,5'-dithiobis-(2-nitrobenzoic acid) 1 mM, 50% inactivation Saccharolobus solfataricus
EDTA 50 mM, 95% inhibition Saccharolobus solfataricus
additional information galactose (0.04 mM), mannose (0.04 mM) and ribose (0.04 mM) do not inhibit glucose oxidation by NAD+ Saccharolobus solfataricus
NADPH inhibits the NAD+-dependentcarbohydrate oxidations in a competitive manner with respect to NAD+ Saccharolobus solfataricus
NEM 3 mM, 2 h, 50% inactivation Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 NADP+ pH 9.0, 70°C, cosubstrate: beta-D-glucose Saccharolobus solfataricus
0.03
-
 NADP+ pH 9.0, 70°C, cosubstrate: D-galactose Saccharolobus solfataricus
0.03
-
 NADP+ pH 9.0, 70°C, cosubstrate: D-xylose Saccharolobus solfataricus
0.44
-
 beta-D-glucose pH 9.0, 70°C, cosubstrate: NADP+ Saccharolobus solfataricus
1.2
-
 NAD+ pH 9.0, 70°C, cosubstrate: beta-D-glucose Saccharolobus solfataricus
1.2
-
 NAD+ pH 9.0, 70°C, cosubstrate: D-xylose Saccharolobus solfataricus
2.2
-
 D-xylose pH 9.0, 70°C, cosubstrate: NADP+ Saccharolobus solfataricus
8
-
 beta-D-glucose pH 9.0, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus
22
-
 D-galactose pH 9.0, 70°C, cosubstrate: NADP+ Saccharolobus solfataricus
68
-
 D-xylose pH 9.0, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 20 mM, maximal activation of enzyme inactivated by dialysis against Mg2+-free 20 mM-triethanolamine/HCl buffer, pH 7.0 Saccharolobus solfataricus
Mg2+ 20 mM, maximal activation of enzyme inactivated by dialysis against Mg2+-free 20 mM-triethanolamine/HCl buffer, pH 7.0 Saccharolobus solfataricus
Mn2+ 20 mM, maximal activation of enzyme inactivated by dialysis against Mg2+-free 20 mM-triethanolamine/HCl buffer, pH 7.0 Saccharolobus solfataricus
additional information Ni2+, Cd2+ and univalent cations are ineffective in promoting enzyme reactivation Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 gel filtration in presence of 5% SDS Saccharolobus solfataricus
60000
-
 gel filtration in presence of 6 M guanidinium chloride Saccharolobus solfataricus
124000
-
 gel filtration Saccharolobus solfataricus
130000
-
 equilibrium-sedimentation Saccharolobus solfataricus

Organic Solvent Stability

Organic Solvent Comment Organism
Ethylene glycol preparations became inactivated irreversibly when stored in the absence of ethylene glycol Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus O93715
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
437
-
 pH 9.0, 70°C Saccharolobus solfataricus

Storage Stability

Storage Stability Organism
37°C, protein concentration 0.2 mg/ml, 50% of the activity is lost after 40 days Saccharolobus solfataricus
4°C, 20 mM MgCl2 and 20% (v/v) ethylene glycol, stable for several months Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + NADP+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
6-deoxy-D-glucose + NAD+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
beta-D-glucose + NAD+ + H2O when NAD+ serves as electron acceptor, D-glucose is oxidized at the highest rate among the substrates tested. No reverse reaction is evident, under various reaction conditions, when gluconic acid is used as substrate Saccharolobus solfataricus D-gluconate + NADH + 2 H+
-
 ir
beta-D-glucose + NADP+ + H2O it is likely that the first product of this reaction is delta-gluconolactone, which is readily converted into the acid. The lactone hydrolysis is facilitated by the high temperature and the slight alkaline pH value of the standard reaction conditions, thus explaining why no reverse reaction is observed under these conditions when delta-gluconolactone is used as substrate. A very slow NADH-dependent reduction of delta-gluconolactone by glucose dehydrogenase is observed at pH 7.0 and 37°C Saccharolobus solfataricus D-gluconate + NADPH + 2 H+
-
 ?
D-altrose + NADP+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
D-galactose + NADP+ + H2O
-
 Saccharolobus solfataricus D-galactonate + NADPH + 2 H+
-
 ?
D-xylose + NAD+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
D-xylose + NADP+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Saccharolobus solfataricus
77
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 protein concentration 0.2 mg/ml, 50% of the activity is lost after 40 days Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Saccharolobus solfataricus
9
-
 assay at, measured at room temperature Saccharolobus solfataricus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 the enzyme stability does not vary significantly in the pH range 5-9 Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NAD+ when NAD+ serves as electron acceptor, D-glucose is oxidized at the highest rate among the substrates tested Saccharolobus solfataricus
NADP+ by using NADP+ as coenzyme D-xylose and 2-deoxy-D-glucose are oxidized at the highest rate Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.075
-
 NADPH pH 9.0, 70°C, substrate: glucose Saccharolobus solfataricus