Crystallization (Comment) | Organism |
---|---|
crystal structure determination and analysis of human 3alpha-HSD3-NADP+-5alpha-androstane-3,17-dione/epiandrosterone complex, which was obtained by co-crystallization with 5alpha-DHT in the presence of NADP+. Although 5alpha-DHT is introduced during the crystallization, oxidoreduction of 5alpha-DHT occurs. The locations of 5alpha-androstane-3,17-dione and epiandrosterone are identified in the steroid-binding sites of two 3alpha-HSD3 molecules per crystal asymmetric unit. 5alpha-androstane-3,17-dione and epiandrosterone are oriented upside-down and flipped relative to each other, providing structural clues for 5alpha-DHT reverse binding in the enzyme with the generation of different products. determination and analysis of human 3alpha-HSD3-NADP+-4-androstene-3,17-dione complex crystal structure | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | when a specific siRNA is used to suppress 3alpha-HSD3 expression without interfering with 3alpha-HSD4, which shares a highly homologous active site, the 5alpha-DHT concentration increases, whereas MCF-7 cell growth is suppressed. Downregulation of 3alpha-HSD3 decreases MCF-7 breast cancer cell growth | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-dihydrotestosterone + NADPH + H+ | Homo sapiens | physiological inactivation of the most potent androgen 5alpha dihydrotestosterone | 3alpha-androstanediol + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P52895 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
breast cancer cell | - |
Homo sapiens | - |
MCF-7 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-dihydrotestosterone + NADPH + H+ | - |
Homo sapiens | 3alpha-androstanediol + NADP+ | - |
r | |
5alpha-dihydrotestosterone + NADPH + H+ | physiological inactivation of the most potent androgen 5alpha dihydrotestosterone | Homo sapiens | 3alpha-androstanediol + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
3alpha-HSD3 | - |
Homo sapiens |
3alpha-hydroxysteroid dehydrogenase type 3 | - |
Homo sapiens |
AKR1C2 | gene name, UniProt | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | downregulation of 3alpha-HSD3 decreases MCF-7 breast cancer cell growth | Homo sapiens |
physiological function | 3alpha-hydroxysteroid dehydrogenase type 3 plays an essential role in the inactivation of the most potent androgen 5alpha-dihydrotestosterone | Homo sapiens |