Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, about 50% inhibition | Mycobacterium tuberculosis | |
EDTA | about 35% inhibition at 1 mM, about 25% inhibition at 10 mM | Mycobacterium tuberculosis | |
K+ | about 30% inhibition at 1 mM, about 20% inhibition at 10 mM | Mycobacterium tuberculosis | |
Ni2+ | 1 mM, about 60% inhibition | Mycobacterium tuberculosis | |
Zn2+ | 1 mM, about 60% inhibition | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
29.5 | - |
NAD+ | pH 9.5, 30°C | Mycobacterium tuberculosis | |
43.5 | - |
(S)-3-hydroxybutyryl-CoA | pH 9.5, 30°C | Mycobacterium tuberculosis | |
50 | - |
NADH | pH 7.0, 30°C | Mycobacterium tuberculosis | |
65.6 | - |
3-acetoacetyl-CoA | pH 7.0, 30°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | enhances activity at 1 mM or 10 mM | Mycobacterium tuberculosis | |
Mg2+ | enhances activity at 1 mM or 10 mM | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxybutyryl-CoA + NAD+ | Mycobacterium tuberculosis | the enzyme is involved in betaq-oxidation cycle | 3-acetoacetyl-CoA + NADH + H+ | - |
r | |
(S)-3-hydroxybutyryl-CoA + NAD+ | Mycobacterium tuberculosis ATCC 25618 | the enzyme is involved in betaq-oxidation cycle | 3-acetoacetyl-CoA + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WNP7 | - |
- |
Mycobacterium tuberculosis ATCC 25618 | P9WNP7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxybutyryl-CoA + NAD+ | the enzyme is involved in betaq-oxidation cycle | Mycobacterium tuberculosis | 3-acetoacetyl-CoA + NADH + H+ | - |
r | |
(S)-3-hydroxybutyryl-CoA + NAD+ | no activity woth NADP+ | Mycobacterium tuberculosis | 3-acetoacetyl-CoA + NADH + H+ | - |
r | |
(S)-3-hydroxybutyryl-CoA + NAD+ | the enzyme is involved in betaq-oxidation cycle | Mycobacterium tuberculosis ATCC 25618 | 3-acetoacetyl-CoA + NADH + H+ | - |
r | |
(S)-3-hydroxybutyryl-CoA + NAD+ | no activity woth NADP+ | Mycobacterium tuberculosis ATCC 25618 | 3-acetoacetyl-CoA + NADH + H+ | - |
r | |
3-acetoacetyl-CoA + NADH + H+ | - |
Mycobacterium tuberculosis | (S)-3-hydroxybutyryl-CoA + NAD+ | - |
r | |
3-acetoacetyl-CoA + NADH + H+ | - |
Mycobacterium tuberculosis ATCC 25618 | (S)-3-hydroxybutyryl-CoA + NAD+ | - |
r | |
3-acetoacetyl-CoA + NADPH + H+ | no dehydration of beta-hydroxybutyryl-CoA to acetoacetyl-CoA with NADP+ as cofactor | Mycobacterium tuberculosis | (S)-3-hydroxybutyryl-CoA + NAD+ | - |
ir | |
3-acetoacetyl-CoA + NADPH + H+ | no dehydration of beta-hydroxybutyryl-CoA to acetoacetyl-CoA with NADP+ as cofactor | Mycobacterium tuberculosis ATCC 25618 | (S)-3-hydroxybutyryl-CoA + NAD+ | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
FadB2 | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
NAD+-dependent dehydration of beta-hydroxybutyryl-CoA to acetoacetyl-CoA | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
NAD+ | pH 9.5, 30°C | Mycobacterium tuberculosis | |
0.011 | - |
3-acetoacetyl-CoA | pH 7.0, 30°C | Mycobacterium tuberculosis | |
0.012 | - |
(S)-3-hydroxybutyryl-CoA | pH 9.5, 30°C | Mycobacterium tuberculosis | |
0.189 | - |
NADH | pH 7.0, 30°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6.5 | conversion of acetoacetyl-CoA to beta-hydroxybutyryl-CoA decreases above pH 6.5 | Mycobacterium tuberculosis |
10 | - |
NAD+-dependent dehydration of beta-hydroxybutyryl-CoA to acetoacetyl-CoA | Mycobacterium tuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9 | 11 | pH 9.0: about 45% of maximal activity, pH 11.0: about 55% of maximal activity | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | no dehydration of beta-hydroxybutyryl-CoA to acetoacetyl-CoA with NADP+ as cofactor | Mycobacterium tuberculosis | |
NADH | conversion of acetoacetyl-CoA to beta-hydroxybutyryl-CoA | Mycobacterium tuberculosis | |
NADPH | conversion of acetoacetyl-CoA to beta-hydroxybutyryl-CoA | Mycobacterium tuberculosis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Mycobacterium tuberculosis | calculated, native protein | - |
5.2 |
Mycobacterium tuberculosis | calculated, recombinant protein | - |
6.1 |
General Information | Comment | Organism |
---|---|---|
malfunction | a null mutant of fadB2 shows no significant differences from the wild-type strain with regard to lipid composition, utilization of different fatty acid carbon sources and tolerance to various stresses | Mycobacterium tuberculosis |
metabolism | the enzyme is involved in beta-oxidation cycle | Mycobacterium tuberculosis |