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Literature summary for 1.1.1.35 extracted from

  • Osumi, T.; Hashimoto, T.
    Purification and properties of mitochondrial and peroxisomal 3-hydroxyacyl-CoA dehydrogenase from rat liver (1980), Arch. Biochem. Biophys., 203, 372-383.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0054
-
NADH
-
Rattus norvegicus
0.0163
-
(S)-3-hydroxyoctanoyl-CoA mitochondrial enzyme, oxidation of (S)-3-hydroxyoctanoyl-CoA Rattus norvegicus
0.0169
-
acetoacetyl-CoA
-
Rattus norvegicus
0.0286
-
(S)-3-hydroxyhexanoyl-CoA mitochondrial enzyme, oxidation of (S)-3-hydroxyhexanoyl-CoA Rattus norvegicus
0.0585
-
NAD+
-
Rattus norvegicus
0.0699
-
(S)-3-hydroxybutyryl-CoA mitochondrial enzyme, oxidation of (S)-3-hydroxybutyryl-CoA Rattus norvegicus
0.088
-
(S)-3-hydroxydecanoyl-CoA mitochondrial enzyme, oxidation of (S)-3-hydroxydecanoyl-CoA Rattus norvegicus
0.435
-
NADPH
-
Rattus norvegicus
0.56
-
acetoacetyl-pantetheine
-
Rattus norvegicus
11
-
NADP+
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-
peroxisome
-
Rattus norvegicus 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
2 * 32000, mitochondrial enzyme, SDS-PAGE Rattus norvegicus
65000
-
mitochondrial enzyme, gel filtration Rattus norvegicus
70000
-
peroxisomal enzyme, gel filtration Rattus norvegicus
77000
-
1 * 77000, peroxisomal enzyme, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-3-hydroxybutyryl-CoA + NAD+ Rattus norvegicus
-
acetoacetyl-CoA + NADH
-
r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
calcium phosphate gel, ammonium sulfate, CM-Sephadex, Blue Dextran-Sepharose 4B, calcium phosphate gel-cellulose, Blue Dextran-Sepharose 4B, mitochondrial enzyme Rattus norvegicus
phosphocellulose, ammonium sulfate, CM-cellulose, ammonium sulfate, peroxisomal enzyme Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.6
-
peroxisomal enzyme Rattus norvegicus
565
-
mitochondrial enzyme Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, 120 mM potassium phosphate, pH 7.0, several months without loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxybutyryl-CoA + NAD+
-
Rattus norvegicus acetoacetyl-CoA + NADH
-
r
(S)-3-hydroxybutyryl-CoA + NADP+ low activity for mitochondrial enzyme Rattus norvegicus acetoacetyl-CoA + NADPH
-
r
(S)-3-hydroxydecanoyl-CoA + NAD+
-
Rattus norvegicus 3-ketodecanoyl-CoA + NADH
-
r
(S)-3-hydroxyhexanoyl-CoA + NAD+
-
Rattus norvegicus 3-oxohexanoyl-CoA + NADH + H+
-
r
3-ketooctanoyl-CoA + NADH
-
Rattus norvegicus (S)-3-hydroxyoctanoyl-CoA + NAD+
-
?
acetoacetyl-pantetheine + NADH
-
Rattus norvegicus (S)-3-hydroxybutyryl-pantetheine
-
?

Subunits

Subunits Comment Organism
dimer 2 * 32000, mitochondrial enzyme, SDS-PAGE Rattus norvegicus
monomer 1 * 77000, peroxisomal enzyme, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
mitochondrial enzyme, reduction of acetoacetyl-CoA Rattus norvegicus
6.2
-
peroxisomal enzyme, reduction of acetoacetyl-CoA Rattus norvegicus
9.6
-
mitochondrial enzyme, oxidation of (S)-3-hydroxybutyryl-CoA Rattus norvegicus
9.8
-
peroxisomal enzyme, oxidation of (S)-3-hydroxybutyryl-CoA Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rattus norvegicus
NADP+
-
Rattus norvegicus