Literature summary for 1.1.1.347 extracted from

Noge, K.; Kato, M.; Mori, N.; Kataoka, M.; Tanaka, C.; Yamasue, Y.; Nishida, R.; Kuwahara, Y.
Geraniol dehydrogenase, the key enzyme in biosynthesis of the alarm pheromone, from the astigmatid mite Carpoglyphus lactis (Acari: Carpoglyphidae) (2008), FEBS J., 275, 2807-2817.

Search for more literature for 1.1.1.347

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.051	-	geraniol	in 50 mM Tris-HCl (pH 8.5), at 22°C	Carpoglyphus lactis
0.0595	-	NAD+	in 50 mM Tris-HCl (pH 8.5), at 22°C	Carpoglyphus lactis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40630	-	1 * 40630, calculated from amino acid sequence	Carpoglyphus lactis
42800	-	1 * 42800, active form, SDS-PAGE	Carpoglyphus lactis
56200	-	gel filtration	Carpoglyphus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
geraniol + NAD+	Carpoglyphus lactis	the enzyme selectively oxidizes geraniol	geranial + NADH + H+	-	ir

Organism

Organism	UniProt	Comment	Textmining
Carpoglyphus lactis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, Toyopearl phenyl-650M column chromatography, Toyopearl HW-50F column chromatography, DEAE-cellulose column chromatography, Blue-Cellulofine column chromatography, MonoQ column chromatography and Superdex 200gel filtration	Carpoglyphus lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.016	-	crude enzyme, in 50 mM Tris-HCl (pH 8.5), at 22°C	Carpoglyphus lactis
31	-	after 1879fold purification, in 50 mM Tris-HCl (pH 8.5), at 22°C	Carpoglyphus lactis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesol + NAD+	50% activity compared to geraniol	Carpoglyphus lactis	(2E,6E)-farnesyl aldehyde + NADH + H+	-	?
3-methyl-2-buten-1-ol + NAD+	4.3% activity compared to geraniol	Carpoglyphus lactis	3-methyl-but-2-enal + NADH + H+	-	?
citronellol + NAD+	4.3% activity compared to geraniol	Carpoglyphus lactis	citronellal + NADH + H+	-	?
geraniol + NAD+	the enzyme selectively oxidizes geraniol	Carpoglyphus lactis	geranial + NADH + H+	-	ir
additional information	NADP+ is ineffective as a cofactor. The enzyme does not use nerol as substrate. Straight-chain alcohols such as 1-octanol, 2-buten-1-ol, 1-butanol and ethanol serve as poor substrates	Carpoglyphus lactis	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 40630, calculated from amino acid sequence	Carpoglyphus lactis
monomer	1 * 42800, active form, SDS-PAGE	Carpoglyphus lactis

Synonyms

Synonyms	Comment	Organism
GeDH	-	Carpoglyphus lactis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	-	Carpoglyphus lactis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2996	-	geraniol	in 50 mM Tris-HCl (pH 8.5), at 22°C	Carpoglyphus lactis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Carpoglyphus lactis

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	10	as the pH increases from 7.0 to 7.5, there is a sharp increase in enzyme activity whereas the activity decreases sharply beyond pH 10.0	Carpoglyphus lactis

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	NADP+ is ineffective as cofactor	Carpoglyphus lactis

General Information

General Information	Comment	Organism
physiological function	the enzyme plays an important role in the biosynthesis of neral, an alarm pheromone in Carpoglyphus lactis	Carpoglyphus lactis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
58800	-	geraniol	in 50 mM Tris-HCl (pH 8.5), at 22°C	Carpoglyphus lactis

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Release 2023.1 (January 2023)