Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.345 extracted from

  • Bonete, M.J.; Ferrer, J.; Pire, C.; Penades, M.; Ruiz, J.L.
    2-Hydroxyacid dehydrogenase from Haloferax mediteranii, a D-isomer-specific member of the 2-dehydrogenase family (2000), Biochimie, 82, 1143-1150.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
NADH with 2-oxobutyrate as cosubstrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C Haloferax mediterranei
0.086
-
NADH with 2-oxoisocaproate as cosubstrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C Haloferax mediterranei
0.096
-
NADH with pyruvate as cosubstrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C Haloferax mediterranei
0.14
-
D-lactate in 0.1 M acetate buffer, pH 5.0, 4 M NaCl, at 52°C Haloferax mediterranei
0.36
-
2-oxobutyrate in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C Haloferax mediterranei
0.56
-
pyruvate in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C Haloferax mediterranei
2.3
-
2-oxoisocaproate in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C Haloferax mediterranei

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl maximum activity in the presence of 4 M NaCl Haloferax mediterranei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59500
-
2 * 59500, CTAB-PAGE Haloferax mediterranei
62900
-
2 * 62900, SDS-PAGE Haloferax mediterranei
101400
-
gel filtration Haloferax mediterranei

Organism

Organism UniProt Comment Textmining
Haloferax mediterranei
-
-
-
Haloferax mediterranei R4, ATCC 33500
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sepharose column chromatography, DEAE-cellulose column chromatography, Q-Sepharose column chromatography, Sephacryl S-300 gel filtration, and Sephadex G25 gel filtration Haloferax mediterranei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.427
-
with 2-oxoisocaproate as substrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C Haloferax mediterranei
0.799
-
with 2-oxobutyrate as substrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C Haloferax mediterranei
1.462
-
with pyruvate as substrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C Haloferax mediterranei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutyrate + NADH + H+ 55% activity compared to pyruvate Haloferax mediterranei D-2-hydroxybutyrate + NAD+
-
r
2-oxobutyrate + NADH + H+ 55% activity compared to pyruvate Haloferax mediterranei R4, ATCC 33500 D-2-hydroxybutyrate + NAD+
-
r
2-oxoisocaproate + NADH + H+ 29% activity compared to pyruvate Haloferax mediterranei L-2-hydroxyisocaproate + NAD+
-
r
2-oxoisocaproate + NADH + H+ 29% activity compared to pyruvate Haloferax mediterranei R4, ATCC 33500 L-2-hydroxyisocaproate + NAD+
-
r
D-2-hydroxybutyrate + NAD+
-
Haloferax mediterranei 2-oxobutyrate + NADH + H+
-
r
D-2-hydroxybutyrate + NAD+
-
Haloferax mediterranei R4, ATCC 33500 2-oxobutyrate + NADH + H+
-
r
D-lactate + NAD+
-
Haloferax mediterranei pyruvate + NADH + H+
-
r
L-2-hydroxycaproate + NAD+
-
Haloferax mediterranei 2-oxocaproate + NADH + H+
-
r
additional information no significant activity with L-lactate, L-2-hydroxybutyrate, L-hydroxyisocaproate, phenylpyruvate, 2-oxomethyl-n-valerate, and 2-oxoisovalerate Haloferax mediterranei ?
-
?
additional information no significant activity with L-lactate, L-2-hydroxybutyrate, L-hydroxyisocaproate, phenylpyruvate, 2-oxomethyl-n-valerate, and 2-oxoisovalerate Haloferax mediterranei R4, ATCC 33500 ?
-
?
pyruvate + NADH + H+ 100% activity Haloferax mediterranei D-lactate + NAD+
-
r
pyruvate + NADH + H+ 100% activity Haloferax mediterranei R4, ATCC 33500 D-lactate + NAD+
-
r

Subunits

Subunits Comment Organism
homodimer 2 * 59500, CTAB-PAGE Haloferax mediterranei
homodimer 2 * 62900, SDS-PAGE Haloferax mediterranei

Synonyms

Synonyms Comment Organism
NAD-dependent D-2-hydroxyacid dehydrogenase
-
Haloferax mediterranei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
52
-
-
Haloferax mediterranei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
65 75 above 60°C there is a pronounced activity decrease, while there is nearly no activity at 75°C Haloferax mediterranei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
optimum pH for reaction with pyruvate or 2-oxobutyrate Haloferax mediterranei
7.5 8.5 optimum pH values for reaction with 2-oxoisocaproate Haloferax mediterranei

pH Range

pH Minimum pH Maximum Comment Organism
5 9
-
Haloferax mediterranei

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme is strictly NAD+-dependent Haloferax mediterranei