Application | Comment | Organism |
---|---|---|
drug development | a yeast expression system can serve to study the influence of selected mutations in human HMG-CoA reductase on the sensitivity of the enzyme to commonly prescribed statins, thus this model system is suitable for the development and selection of lipid-lowering drugs as well as for the examination of DNA sequence variations in the context of statin therapy | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
cDNA encoding the wild-type and mutant forms of human HMG-CoA reductase expressed under control of the yeast MET25 promoter in a Saccharomyces cerevisiae strain with deletions of both HMG1 and HMG2 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of human HMG-CoA reductase in yeast complements the lethal phenotype of Saccharomyces cerevisiae strains lacking the HMG1 and HMG2 genes | Homo sapiens |
Q766H | restores viability of Saccharomyces cerevisiae strains lacking the HMG1 and HMG2 genes, thus is catalytically active in yeast cells. Q766H mutation, which affects the structure of the catalytic domain, increases the sensitivity of the enzyme towards statin treatment | Homo sapiens |
R393Q | restores viability of Saccharomyces cerevisiae strains lacking the HMG1 and HMG2 genes, thus is catalytically active in yeast cells. R393Q mutation does not change the properties of the enzyme towards statin treatment | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
atorvastatin | has a differentiating effect on wild-type and mutant forms of the human protein | Homo sapiens | |
fluvastatin | has a differentiating effect on wild-type and mutant forms of the human protein | Homo sapiens | |
simvastatin | does not allow differentiation between the wild-type and mutant forms of the human protein | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood | samples of patients with cardiovascular disorders | Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
HMG-CoA reductase | - |
Homo sapiens |
HMG1 | - |
Homo sapiens |
HMG2 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens |