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Literature summary for 1.1.1.34 extracted from

  • Montalvetti, A.; Pena-Diaz, J.; Hurtado, R.; Ruiz-Perez, L.M.; Gonzales-Pacanowska, D.
    Characterization and regulation of Leishmania major 3-hydroxy-3-methylglutaryl-CoA reductase (2000), Biochem. J., 349, 27-34.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.34

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Leishmania major

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 (R,S)-3-hydroxy-3-methylglutaryl-CoA
-
 Leishmania major
0.081
-
 NADPH
-
 Leishmania major

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 alpha4, 4 * 46000, SDS-PAGE Leishmania major
184000
-
 gel filtration Leishmania major

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxy-3-methylglutaryl-CoA + NADPH Leishmania major
-
 (R)-mevalonate + CoA + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Leishmania major
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Leishmania major

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0335
-
-
 Leishmania major

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxy-3-methylglutaryl-CoA + NADPH
-
 Leishmania major (R)-mevalonate + CoA + NADP+
-
 ?

Subunits

Subunits Comment Organism
tetramer alpha4, 4 * 46000, SDS-PAGE Leishmania major

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Leishmania major