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Literature summary for 1.1.1.336 extracted from
- UDP-N-acetyl-D-mannosamine dehydrogenase from Escherichia coli (1982), Methods Enzymol., 83, 519-522.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | 2 mM, maximum activation | Escherichia coli |  |
| additional information | the presence of SH compounds is required for activity | Escherichia coli | |
| N-acetylglucosamine 1-phosphate | activation at low UDP-N-acetylmannosamine concentrations | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
UDP-N-acetyl-alpha-D-mannosamine | pH 10.0, 37°C, presence of 150 mM KCl | Escherichia coli | |
| 0.21 | - |
NAD+ | pH 10.0, 37°C | Escherichia coli | |
| 0.22 | - |
UDP-N-acetyl-alpha-D-mannosamine | pH 10.0, 37°C, presence of 1 mM N-acetylglucosamine 1-phosphate | Escherichia coli | |
| 0.38 | - |
UDP-N-acetyl-alpha-D-mannosamine | pH 10.0, 37°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | activation at low UDP-N-acetylmannosamine concentrations | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE | Escherichia coli |
| 100000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C, 20 mM Tris-HCl, pH 7.9, 3 M KC1, 2 mM 2-mercaptoethanol, and 10% glycerol, storage without loss of activity for 2 weeks, presence of bovine serum albumin stabilizes | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-mannosamine + 2 NAD+ + H2O | enzyme is strictly specific for UDP-N-acetyl-mannosamine and NAD + | Escherichia coli | UDP-N-acetyl-alpha-D-mannosaminuronate + 2 NADH + 2 H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | 10 | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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