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Literature summary for 1.1.1.336 extracted from

  • Namboori, S.C.; Graham, D.E.
    Acetamido sugar biosynthesis in the Euryarchaea (2008), J. Bacteriol., 190, 2987-2996.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.336

Activating Compound

Activating Compound Comment Organism Structure
tris-(2-carboxyethyl)phosphine hydrochloride 1 mM, about 25% increase in activity Methanococcus maripaludis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanococcus maripaludis

Inhibitors

Inhibitors Comment Organism Structure
N-acetylglucosamine 1.3 mM, 50% inhibition Methanococcus maripaludis
N-acetylmannosamine 0.07 mM, 50% inhibition Methanococcus maripaludis
UDP 0.5 mM, 50% inhibition Methanococcus maripaludis
UDP-glucose 0.6 mM, 50% inhibition Methanococcus maripaludis
UTP 1 mM, 50% inhibition Methanococcus maripaludis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.57
-
 NAD+ pH 8.5, 37°C Methanococcus maripaludis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information salts or divalent cations are not required Methanococcus maripaludis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 4 * 50000, SDS-PAGE, 4 * 51400, calculated, including His-tag Methanococcus maripaludis
51400
-
 4 * 50000, SDS-PAGE, 4 * 51400, calculated, including His-tag Methanococcus maripaludis
200000
-
 gel filtration Methanococcus maripaludis

Organism

Organism UniProt Comment Textmining
Methanococcus maripaludis Q6LZC3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrate: UDP-glucose or N-acetyl-D-mannosamine Methanococcus maripaludis ?
-
 ?
UDP-N-acetyl-alpha-D-mannosamine + 2 NAD+ + H2O
-
 Methanococcus maripaludis UDP-N-acetyl-alpha-D-mannosaminuronate + 2 NADH + 2 H+ ratio of 1.95 NAD molecules consumed per UDP-N-acetyl-alpha-D-mannosaminuronate molecule produced, 4-electron oxidation ?

Subunits

Subunits Comment Organism
tetramer 4 * 50000, SDS-PAGE, 4 * 51400, calculated, including His-tag Methanococcus maripaludis

Synonyms

Synonyms Comment Organism
MMP0706
-
 Methanococcus maripaludis
wecC
-
 Methanococcus maripaludis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
 NAD+ pH 8.5, 37°C Methanococcus maripaludis

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor: NADP+ Methanococcus maripaludis
NAD+
-
 Methanococcus maripaludis