Application | Comment | Organism |
---|---|---|
additional information | plant (4aR,7S,7aS)-nepetalactone can be used for synthesis of (4aR,7S,7aS)-nepetalactol, an aphid pheromone, useful in aphid control | Nepeta racemosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 79% inhibition at 1.0 mM, 87% at 10 mM | Nepeta racemosa | |
4-chloromercuribenzoate | complete inhibition at 1 mM and above | Nepeta racemosa | |
EDTA | 44% inhibition at 100 mM | Nepeta racemosa | |
iodoacetamide | 5% inhibition at 1.0 mM, 22% at 10 mM | Nepeta racemosa | |
NEM | 55% inhibition at 1.0 mM, complete inhibition at 10 mM | Nepeta racemosa | |
Triton X-100 | potent inhibition at 0.01-0.1% | Nepeta racemosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
NADP+ | with 8-hydroxynerol, pH 9.5, 25°C | Nepeta racemosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Nepeta racemosa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | the enzyme contains zinc ions | Nepeta racemosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
1 * 40000 + 1 x 42000, SDS-PAGE | Nepeta racemosa |
63000 | - |
gel filtration | Nepeta racemosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6E)-8-hydroxygeraniol + 2 NADP+ | Nepeta racemosa | - |
(6E)-8-oxogeranial + 2 NADPH + 2 H+ | - |
? | |
(6E)-8-hydroxynerol + 2 NADP+ | Nepeta racemosa | - |
? + 2 NADPH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nepeta racemosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 1149fold from leaves to homogeneity by ultracentrifugation, ammonium sulfate fractionation, hydrophobic inetraction and anion exchange chromatography, affinity chromatography, and gel filtration | Nepeta racemosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nepeta racemosa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1417 | - |
purified enzyme, pH 9.5, 25°C | Nepeta racemosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6E)-8-hydroxygeraniol + 2 NADP+ | - |
Nepeta racemosa | (6E)-8-oxogeranial + 2 NADPH + 2 H+ | - |
? | |
(6E)-8-hydroxygeraniol + 2 NADP+ | oxidization at C1 and C8, depending on numbering of the molecule, GC-MS product analysis | Nepeta racemosa | (6E)-8-oxogeranial + 2 NADPH + 2 H+ | - |
? | |
(6E)-8-hydroxynerol + 2 NADP+ | - |
Nepeta racemosa | ? + 2 NADPH + 2 H+ | - |
? | |
(6E)-8-hydroxynerol + 2 NADP+ | oxidization at C1 and C8, depending on numbering of the molecule, GC-MS product analysis | Nepeta racemosa | ? + 2 NADPH + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 40000 + 1 x 42000, SDS-PAGE | Nepeta racemosa |
Synonyms | Comment | Organism |
---|---|---|
acyclic monoterpene primary alcohol:NADP+ oxidoreductase | - |
Nepeta racemosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Nepeta racemosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Nepeta racemosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NAD+ | Nepeta racemosa | |
NADP+ | dependent on | Nepeta racemosa |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme plays a role in the biosynthesis of iridoid monoterpenes, e.g. (4aR,7S,7aS)-nepetalactone | Nepeta racemosa |