KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
L-galactose | pH 7.5, temperature not specified in the publication | Pisum sativum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactose + NAD+ | Pisum sativum | a step in the Smirnoff-Wheeler pathway, ascorbate biosynthesis in higher plants | L-galactono-1,4-lactone + NADH + H+ | - |
? | |
L-galactose + NAD+ | Arabidopsis thaliana | a step in the Smirnoff-Wheeler pathway, ascorbate biosynthesis in higher plants | L-galactono-1,4-lactone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Pisum sativum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryonic axis | - |
Pisum sativum | - |
leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactose + NAD+ | - |
Pisum sativum | L-galactono-1,4-lactone + NADH + H+ | - |
? | |
L-galactose + NAD+ | - |
Arabidopsis thaliana | L-galactono-1,4-lactone + NADH + H+ | - |
? | |
L-galactose + NAD+ | a step in the Smirnoff-Wheeler pathway, ascorbate biosynthesis in higher plants | Pisum sativum | L-galactono-1,4-lactone + NADH + H+ | - |
? | |
L-galactose + NAD+ | a step in the Smirnoff-Wheeler pathway, ascorbate biosynthesis in higher plants | Arabidopsis thaliana | L-galactono-1,4-lactone + NADH + H+ | - |
? | |
additional information | no activity with D-galactose, D-glucose, D-mannose, L-fucose and D-arabinose. The enzyme oxidizes L-sorbosone with very low affinity | Pisum sativum | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the reduction activity with NADP+ is 10% of that with NAD+ | Pisum sativum | |
NADP+ | the reduction activity with NADP+ is 10% of that with NAD+ | Pisum sativum |