Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-oxobutyrate | 90% substrate inhibition at 20 mM | Chromohalobacter salexigens | |
additional information | not inhibited by formaldehyde, acetaldehyde, betaine aldehyde, propionaldehyde, DL-glyceraldehyde, phosphonoacetaldehyde, glyoxylate, 2-oxobutyrate, 4-oxobutyrate and 3-sulfopropanaldehyde | Chromohalobacter salexigens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.061 | - |
NADPH | in 0.02 mMTris/H2SO4 buffer, pH 9.0, at 23°C | Chromohalobacter salexigens | |
0.13 | - |
2-sulfoacetaldehyde | in 0.02 mMTris/H2SO4 buffer, pH 9.0, at 23°C | Chromohalobacter salexigens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
SDS-PAGE | Chromohalobacter salexigens |
27100 | - |
estimated from amino acid sequence | Chromohalobacter salexigens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chromohalobacter salexigens | - |
- |
- |
Chromohalobacter salexigens DSM 3043 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Mono Q column chromatography, phenyl Superose gel filtration | Chromohalobacter salexigens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:ammonium-grown cell | IsfD is detected at low levels in extracts of ammonium-grown cells | Chromohalobacter salexigens | - |
culture condition:taurine-grown cell | IsfD is detected at high levels in extracts of taurine-grown cells | Chromohalobacter salexigens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-sulfoacetaldehyde + NADPH + H+ | - |
Chromohalobacter salexigens | isethionate + NADP+ | - |
ir | |
2-sulfoacetaldehyde + NADPH + H+ | - |
Chromohalobacter salexigens DSM 3043 | isethionate + NADP+ | - |
ir | |
4-oxobutyrate + NADPH + H+ | - |
Chromohalobacter salexigens | ? | - |
ir | |
4-oxobutyrate + NADPH + H+ | - |
Chromohalobacter salexigens DSM 3043 | ? | - |
ir | |
additional information | NADH is not a substrate | Chromohalobacter salexigens | ? | - |
? | |
additional information | NADH is not a substrate | Chromohalobacter salexigens DSM 3043 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
isethionate formation, reductase D | - |
Chromohalobacter salexigens |
IsfD | isoform, this enzyme represents the major portion of the IsfD activity described for taurine-grown cells | Chromohalobacter salexigens |
IsfD2 | isoform, this enzyme is responsible for the low activity observed in extracts of ammonium-grown cells | Chromohalobacter salexigens |
NADPH-dependent sulfoacetaldehyde reductase | - |
Chromohalobacter salexigens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
in Tris/H2SO4 buffer | Chromohalobacter salexigens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | IsfD shows a broad pH optimum from about pH 6.5 to pH 9.5. There is a steep increase in activity from pH 4.5 (negligible activity) and an equally steep decrease above pH 9.5 | Chromohalobacter salexigens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | - |
IsfD is stable in Tris/H2SO4 buffer, pH 9.0 | Chromohalobacter salexigens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | highly specific for NADPH. NADH is not a substrate. | Chromohalobacter salexigens |