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Literature summary for 1.1.1.312 extracted from

  • Otsuka, Y.; Nakamura, M.; Shigehara, K.; Sugimura, K.; Masai, E.; Ohara, S.; Katayama, Y.
    Efficient production of 2-pyrone 4,6-dicarboxylic acid as a novel polymer-based material from protocatechuate by microbial function (2006), Appl. Microbiol. Biotechnol., 71, 608-614.
    View publication on PubMed

Application

Application Comment Organism
biotechnology production of 2-pyrone-4,6-dicarboxylic acid from protocatechuate as a precursor for biopolymers Sphingomonas paucimobilis
industry the authors focus on the metabolic intermediate 2-pyrone-4,6-dicarboxylic acid as a potential raw material for novel, bio-based polymers Sphingomonas paucimobilis

Cloned(Commentary)

Cloned (Comment) Organism
coexpression with protocatechuate 4,5-dioxygenase in Pseudomonas putida PpY1100 Sphingomonas paucimobilis
into the pKT230MC vector for expression in Pseudomonas putida PpY1100 and into pUC119 for DNA amplification in Escherichia coli DH5alpha Sphingomonas paucimobilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+ + H2O Sphingomonas paucimobilis
-
4-carboxy-2-hydroxy-cis,cis-muconate + NADPH + 2 H+
-
?
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+ + H2O Sphingomonas paucimobilis SYK-6
-
4-carboxy-2-hydroxy-cis,cis-muconate + NADPH + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Sphingomonas paucimobilis
-
-
-
Sphingomonas paucimobilis
-
SYK-6
-
Sphingomonas paucimobilis SYK-6
-
-
-
Sphingomonas paucimobilis SYK-6
-
SYK-6
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Sphingomonas paucimobilis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+ + H2O
-
Sphingomonas paucimobilis 4-carboxy-2-hydroxy-cis,cis-muconate + NADPH + 2 H+
-
?
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+ + H2O
-
Sphingomonas paucimobilis SYK-6 4-carboxy-2-hydroxy-cis,cis-muconate + NADPH + 2 H+
-
?
4-carboxy-2-hydroxymuconate semialdehyde + NADP+
-
Sphingomonas paucimobilis 2-pyrone-4,6-dicarboxylic acid + NADPH + H2O
-
?
4-carboxy-2-hydroxymuconate semialdehyde + NADP+
-
Sphingomonas paucimobilis SYK-6 2-pyrone-4,6-dicarboxylic acid + NADPH + H2O
-
?
protocatechuate + NADP+ + H2O
-
Sphingomonas paucimobilis 2-pyrone-4,6-dicarboxylic acid + NADPH + H+
-
?
protocatechuate + NADP+ + H2O
-
Sphingomonas paucimobilis SYK-6 2-pyrone-4,6-dicarboxylic acid + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
2-hydroxy-4-carboxymuconate-6-semialdehyde dehydrogenase
-
Sphingomonas paucimobilis
4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase
-
Sphingomonas paucimobilis
CHMS dehydrogenase
-
Sphingomonas paucimobilis
CHMS hydrolase
-
Sphingomonas paucimobilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
fermenter condition for production of 2-pyrone-4,6-dicarboxylic acid, PDC Sphingomonas paucimobilis

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Sphingomonas paucimobilis