Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Scheffersomyces stipitis |
Protein Variants | Comment | Organism |
---|---|---|
K21A | strong preference for NADH over NADPH | Scheffersomyces stipitis |
K21A/N272D | catalytic efficiency is almost 9fold that of the K21A mutant and 2fold that of the wild-type enzyme. Strong preference for NADH over NADPH | Scheffersomyces stipitis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADH | mutant K21A/N272D, pH 6.5, 35°C | Scheffersomyces stipitis | |
0.02 | - |
NADH | wild-type, pH 6.5, 35°C | Scheffersomyces stipitis | |
0.03 | - |
NADH | mutant K21A, pH 6.5, 35°C | Scheffersomyces stipitis | |
0.03 | - |
NADPH | wild-type, pH 6.5, 35°C | Scheffersomyces stipitis | |
33 | - |
D-xylose | wild-type, cosubstrate NADPH, pH 6.5, 35°C | Scheffersomyces stipitis | |
67 | - |
D-xylose | mutant K21A/N272D, cosubstrate NADH, pH 6.5, 35°C | Scheffersomyces stipitis | |
67 | - |
D-xylose | wild-type, cosubstrate NADH, pH 6.5, 35°C | Scheffersomyces stipitis | |
167 | - |
D-xylose | mutant K21A, cosubstrate NADH, pH 6.5, 35°C | Scheffersomyces stipitis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
x * 36000, SDS-PAGE of recombinant enzyme | Scheffersomyces stipitis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Scheffersomyces stipitis | P31867 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Scheffersomyces stipitis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.4 | - |
mutant K21A, substrate NADH, pH 6.5, 35°C | Scheffersomyces stipitis |
5 | - |
mutant K21A, substrate NADPH, pH 6.5, 35°C | Scheffersomyces stipitis |
6.6 | - |
wild-type, substrate NADH, pH 6.5, 35°C | Scheffersomyces stipitis |
20.3 | - |
wild-type, substrate NADPH, pH 6.5, 35°C | Scheffersomyces stipitis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADH + H+ | - |
Scheffersomyces stipitis | xylitol + NAD+ | - |
? | |
D-xylose + NADPH + H+ | - |
Scheffersomyces stipitis | xylitol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36000, SDS-PAGE of recombinant enzyme | Scheffersomyces stipitis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.7 | - |
NADH | mutant K21A, pH 6.5, 35°C | Scheffersomyces stipitis | |
12.7 | - |
NADH | mutant K21A/N272D, pH 6.5, 35°C | Scheffersomyces stipitis | |
13.1 | - |
NADH | wild-type, pH 6.5, 35°C | Scheffersomyces stipitis | |
40.27 | - |
NADPH | wild-type, pH 6.5, 35°C | Scheffersomyces stipitis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
146.8 | - |
NADH | mutant K21A, pH 6.5, 35°C | Scheffersomyces stipitis | |
614 | - |
NADH | wild-type, pH 6.5, 35°C | Scheffersomyces stipitis | |
1271 | - |
NADH | mutant K21A/N272D, pH 6.5, 35°C | Scheffersomyces stipitis | |
1592 | - |
NADPH | wild-type, pH 6.5, 35°C | Scheffersomyces stipitis |